Details of a Researcher - Fujii, Hideki

Reviews, Commentaries, etc. - Fujii, Hideki

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肥満マウスにおけるインフルエンザウイルス感染症の重症化機序の検討

中川知彦, 石井誠, 南宮湖, 藤猪英樹, 浅見貴弘, 八木一馬, 斎藤史武, 藤原宏, 舩津洋平, 石田洋子, 田坂定智, 長谷川直樹, 別役智子

日本呼吸器学会誌 ((一社)日本呼吸器学会) 2 ( 増刊 ) 244 - 244 2013.03

ISSN 2186-5876

インフルエンザウイルス感染におけるクラリスロマイシン主代謝物(14OH-CAM)の投与効果の検討

松井悠輔, 石井誠, 南宮湖, 藤猪英樹, 浅見貴弘, 八木一馬, 斎藤史武, 舩津洋平, 田坂定智, 長谷川直樹, 別役智子

日本呼吸器学会誌 ((一社)日本呼吸器学会) 2 ( 増刊 ) 244 - 244 2013.03

ISSN 2186-5876

インフルエンザウイルス感染およびTLR7/8を介した免疫応答における間葉系幹細胞培養上清の投与効果

浅見貴弘, 石井誠, 藤猪英樹, 南宮湖, 八木一馬, 石田洋子, 舩津洋平, 藤原宏, 斎藤史武, 田坂定智, 長谷川直樹, 別役智子

日本呼吸器学会誌 ((一社)日本呼吸器学会) 2 ( 増刊 ) 249 - 249 2013.03

ISSN 2186-5876

インフルエンザウイルス感染におけるエピジェネティクスの関与の検討

八木一馬, 石井誠, 藤猪英樹, 溝口孝輔, 南宮湖, 浅見貴弘, 石田洋子, 舩津洋平, 藤原宏, 斎藤史武, 田坂定智, 長谷川直樹, 別役智子

日本呼吸器学会誌 ((一社)日本呼吸器学会) 2 ( 増刊 ) 243 - 243 2013.03

ISSN 2186-5876

インフルエンザウイルス感染におけるクラリスロマイシン(CAM)の免疫修飾作用の検討

南宮湖, 石井誠, 藤猪英樹, 若田部誠, 浅見貴弘, 舩津洋平, 君塚善文, 藤原宏, 田坂定智, 長谷川直樹, 別役智子

The Japanese Journal of Antibiotics ((公財)日本感染症医薬品協会) 66 ( Suppl.A ) 14 - 14 2013.03

ISSN 0368-2781

感染症と免疫応答インフルエンザウイルス感染における間葉系幹細胞上清の投与効果の検討

浅見貴弘, 石井誠, 藤猪英樹, 南宮湖, 君塚善文, 舩津洋平, 田坂定智, 長谷川直樹, 別役智子

日本呼吸器学会誌 ((一社)日本呼吸器学会) 1 ( 増刊 ) 129 - 129 2012.03

ISSN 2186-5876

感染症と免疫応答インフルエンザウイルス感染におけるクラリスロマイシンの投与効果の検討

若田部誠, 石井誠, 南宮湖, 藤猪英樹, 浅見貴弘, 舩津洋平, 君塚善文, 田坂定智, 長谷川直樹, 別役智子

日本呼吸器学会誌 ((一社)日本呼吸器学会) 1 ( 増刊 ) 129 - 129 2012.03

ISSN 2186-5876

粘膜/皮膚免疫医学応用の視点よりタイトジャンクションを介したランゲルハンス細胞の抗原獲得は実験的ブドウ球菌性熱傷様皮膚症候群において先制的な液性免疫を担う(Langerhans cells confer pre-emptive immunity via antigen capture through tight junctions in experimental staphylococcal scalded skin syndrome)

永尾圭介, 大内健嗣, 久保亮治, 藤猪英樹, 小安重夫, 天谷雅行

日本免疫学会総会・学術集会記録 ((NPO)日本免疫学会) 40 184 - 184 2011.11

ISSN 0919-1984

Langerhans cells play an essential role in inducing protective humoral immune response subsequent to antigen uptake through tight junctions

Takeshi Ouchi, Akiharu Kubo, Takeya Adachi, Tetsuro Kobayashi, Daniela Y. Kitashima, Hideki Fujii, Bjoern E. Clausen, Shigeo Koyasu, Masayuki Amagai, Keisuke Nagao

JOURNAL OF INVESTIGATIVE DERMATOLOGY (NATURE PUBLISHING GROUP) 131 S83 - S83 2011.09

ISSN 0022-202X

粘膜免疫粘膜免疫系に特徴的な細胞群とその機能脂肪組織関連のナチュラルヘルパー細胞によるTh2サイトカインの先天産生(Innate production of Th2 cytokines by adipose tissue-associated natural helper cell)

茂呂和世, 山田健人, 伊川友活, 河本宏, 藤猪英樹, 小安重夫

日本免疫学会総会・学術集会記録 ((NPO)日本免疫学会) 39 39 - 39 2009.11

ISSN 0919-1984

フォスフォイノシタイド3キナーゼとイムノグロブリンクラススイッチ

西村知泰, 藤猪英樹, 小安重夫

日本生化学会大会プログラム・講演要旨集 ((公社)日本生化学会) 82回 4T4a - 4 2009.09

不活化インフルエンザウイルス粒子に含まれる白血球数減少活性の解析

山本紀一, 阿戸学, 藤猪英樹, 高橋宜聖, 橋本修一, 加地友弘, 竹森利忠

日本免疫学会総会・学術集会記録 ((NPO)日本免疫学会) 35 179 - 179 2005.11

ISSN 0919-1984

HIVnef発現により誘導される成熟T細胞の機能低下

藤猪英樹, 阿戸学, 高橋宜聖, 橋本修一, 中山俊憲, 谷口克, 小安重夫, 竹森利忠

日本免疫学会総会・学術集会記録 ((NPO)日本免疫学会) 35 178 - 178 2005.11

ISSN 0919-1984

Immunological detection of severe acute respiratory syndrome coronavirus by monoclonal antibodies

K Ohnishi, M Sakaguchi, T Kaji, K Akagawa, T Taniyama, M Kasai, Y Tsunetsugu-Yokota, M Oshima, K Yamamoto, N Takasuka, SI Hashimoto, M Ato, H Fujii, Y Takahashi, S Morikawa, K Ishii, T Sata, H Takagi, S Itamura, T Odagiri, T Miyamura, Kurane, I, M Tashiro, T Kurata, H Yoshikura, T Takemori

JAPANESE JOURNAL OF INFECTIOUS DISEASES (NATL INST INFECTIOUS DISEASES) 58 ( 2 ) 88 - 94 2005.04

ISSN 1344-6304

In order to establish immunological detection methods for severe acute respiratory syndrome coronavirus (SARS-CoV), we established monoclonal antibodies directed against structural components of the virus. B cell hybridomas were generated from mice that were hyper-immunized with inactivated SARS-CoV virion. By screening 2,880 generated hybridomas, we established three hybridoma clones that secreted antibodies specific for nucleocapsid protein (N) and 27 clones that secreted antibodies specific for spike protein (S). Among these, four S-protein specific antibodies had in vitro neutralization activity against SARS-CoV infection. These monoclonal antibodies enabled the immunological detection of SARS-CoV by immunofluorescence staining, Western blot or immunohistology. Furthermore, a combination of monoclonal antibodies with different specificities allowed the establishment of a highly sensitive antigen-capture sandwich ELISA system. These monoclonal antibodies would be a useful tool for rapid and specific diagnosis of SARS and also for possible antibody-based treatment of the disease.

Synthetic double-stranded RNA poly(I : C) combined with mucosal vaccine protects against influenza virus infection

T Ichinohe, Watanabe, I, S Ito, H Fujii, M Moriyama, S Tamura, H Takahashi, H Sawa, J Chiba, T Kurata, T Sata, H Hasegawa

JOURNAL OF VIROLOGY (AMER SOC MICROBIOLOGY) 79 ( 5 ) 2910 - 2919 2005.03

ISSN 0022-538X

The mucosal adjuvant effect of synthetic double-stranded RNA polyriboinosinic polyribocytidylic acid [poly(I:C)] against influenza virus was examined under intranasal coadministration with inactivated hemagglutinin (RA) vaccine in BALB/c mice and was shown to have a protective effect against both nasal-restricted infection and lethal lung infection. Intranasal administration of vaccine from PR8 (H1N1) with poly(I:C) induced a high anti-HA immunoglobulin A (IgA) response in the nasal wash and IgG antibody response in the serum, while vaccination without poly(I:C) induced little response. Intracerebral injection confirmed the safety of poly(I:C). In addition, we demonstrated that administration of poly(I:C) with either A/Beijing (H1N1) or A/Yamagata (H1N1) vaccine conferred complete protection against PR8 challenge in this mouse nasal infection model, suggesting that poly(I:C) possessed cross-protection ability against variant viruses. To investigate the mechanism of the protective effect of poly(I:C), mRNA levels of Toll-like receptors and cytokines were examined in the nasal-associated lymphoid tissue after vaccination or virus challenge. Intranasal administration of HA vaccine with poly(I:C) up-regulated expression of Toll-like receptor 3 and alpha/beta interferons as well as Th1- and Th2-related cytokines. We propose that poly(I:C) is a new effective intranasal adjuvant for influenza virus vaccine.

Access to Document (DOI)

SARSコロナウイルスに対するモノクローナル抗体の確立とウイルス抗原検出法への応用

大西和夫, 阪口雅弘, 加地友弘, 横田恭子, 葛西正孝, 谷山忠義, 赤川清子, 高須賀直美, 橋本修一, 阿戸学, 藤猪英樹, 高橋宜聖, 竹森利忠

日本免疫学会総会・学術集会記録 ((NPO)日本免疫学会) 34 94 - 94 2004.11

ISSN 0919-1984

HIVnef発現により誘導される成熟T細胞の機能異常

藤猪英樹, 中山俊憲, 谷口克, 竹森利忠

日本免疫学会総会・学術集会記録 ((NPO)日本免疫学会) 34 92 - 92 2004.11

ISSN 0919-1984

A subcutaneously injected UV-inactivated SARS coronavirus vaccine elicits systemic humoral immunity in mice

Takasuka N, Fujii H, Takahashi Y, Kasai M, Morikawa S, Itamura S, Ishii K, Sakaguchi M, Ohnishi K, Ohshima M, Hashimoto S, Odagiri T, Tashiro M, Yoshikura H, Takemori T, Tsunetsugu-Yokota Y

Int Immunol. (OXFORD UNIV PRESS) 16 ( 10 ) 1423 - 1430 2004.10

ISSN 0953-8178

The recent emergence of severe acute respiratory syndrome (SARS) was caused by a novel coronavirus, SARS-CoV. It spread rapidly to many countries and developing a SARS vaccine is now urgently required. In order to study the immunogenicity of UV-inactivated purified SARS-CoV virion as a vaccine candidate, we subcutaneously immunized mice with UV-inactivated SARS-CoV with or without an adjuvant. We chose aluminum hydroxide gel (alum) as an adjuvant, because of its long safety history for human use. We observed that the UV-inactivated SARS-CoV virion elicited a high level of humoral immunity, resulting in the generation of long-term antibody secreting and memory B cells. With the addition of alum to the vaccine formula, serum IgG production was augmented and reached a level similar to that found in hyper-immunized mice, though it was still insufficient to elicit serum IgA antibodies. Notably, the SARS-CoV virion itself was able to induce long-term antibody production even without an adjuvant. Anti-SARS-CoV antibodies elicited in mice recognized both the spike and nucleocapsid proteins of the virus and were able to neutralize the virus. Furthermore, the UV-inactivated virion induced regional lymph node T-cell proliferation and significant levels of cytokine production (IL-2, IL-4, IL-5, IFN-gamma and TNF-alpha) upon restimulation with inactivated SARS-CoV virion in vitro. Thus, a whole killed virion could serve as a candidate antigen for a SARS vaccine to elicit both humoral and cellular immunity.

Access to Document (DOI)

HIVnef発現により誘導される成熟T細胞の機能異常

藤猪英樹, 中山俊憲, 谷口克, 竹森利忠

日本免疫学会総会・学術集会記録 ((NPO)日本免疫学会) 33 295 - 295 2003.11

ISSN 0919-1984

角膜アログラフトの長期生着と拒絶に関与する抗原提示細胞の性質

堀純子, 大原國俊, 藤猪英樹, 竹森利忠

日本眼科学会雑誌 ((公財)日本眼科学会) 107 ( 臨増 ) 218 - 218 2003.03

ISSN 0029-0203

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