論文 - 金井　隆典

A case of Crohn's disease which initially presented with diffuse inflammation in the rectum and sigmoid colon

Hibi T., Serizawa H., Tomita M., Suematsu M., Kanai T., Watanabe N., Tashiro Y., Sugino Y., Tsuchiya M.

Digestive Endoscopy 5 （ 2 ） 169 - 174 1993年

ISSN  0915-5635

　概要を見る

<p>This case report describes a female patient with Crohn's disease who had diffuse proctosigmoiditis without a longitudinal ulcer or cobblestone appearance at the initial attack. She was treated with sulfasalazine on the presumptive diagnosis of ulcerative colitis. Two and a half months later, painful ulcers in the oral cavity and a deep longitudinal ulcer in the sigmoid colon were found, and a non-caseous granuloma was revealed in the biopsy specimens taken from the sigmoid colon. A definitive diagnosis of Crohn's disease was established from these findings and treatment with a corticosteroid was effective.</p>

Burkitt's lymphoma of the stomach

Miyaguchi S., Hibi T., Kanai T., Tashiro H., Suematsu M., Guevara F. M., Tsuchiya M.

Endoscopy 25 （ 7 ） 494 - 495 1993年

ISSN  0013-726X

A case of Burkitt's lymphoma of the stomach with unusual features [3]

Miyaguchi S., Hibi T., Kanai T., Tashiro H., Suematsu M., Guevara F. M., Tsuchiya M.

Endoscopy 24 （ 6 ） 603 1992年

ISSN  0013-726X

Establishment of epithelial cell lines from human and mouse thymus immortalized by the 12S adenoviral E1a gene product

Hibi T., Fujisawa T., Kanai T., Akatsuka A., Habu S., Handa H., Tsuchiya M.

Thymus 18 （ 3 ） 155 - 167 1991年

ISSN  0165-6090

　概要を見る

<p>To understand the role of thymic epithelial cells in the development of immature thymocytes, the establishment and cloning of thymic epithelial cell lines must be required. In the present study, we established human and mouse thymic epithelial cell lines through the immortalization by the transfection of cDNA sequences of adenoviral E1a 12S mRNA. This procedure resulted in the isolation of five stable cell lines (one human cell line and four mouse cell lines). These cell lines were positive in cytokeratin demonstrated by immunohistochemistry. Electron microscopic study revealed that they had bundles of tonofilaments and desmosome-like tight junctions. These findings indicate that the cell lines immortalized by E1a gene have retained the properties of epithelial cells. MHC class II antigens were not expressed on these cell lines. When interferon-γ was added to the cultured epithelial cell lines, MHC class II antigens were induced in their cytoplasm and on their surface membrane, demonstrated by immunohistochemical and immunofluorescent studies. It is suggested that these stable cell lines from human and mouse thymus might serve a good tool for the further study of thymocytes differentiation and of unknown cytokines derived from thymus epithelium.</p>

A case of enterocolitis with diffuse multiple aphthoid lesions in which non-caseous granulomas were demonstrated microscopically

Serizawa H., Hibi T., Niwa H., Tashiro H., Kanai T., Miura S., Sugino Y., Tashiro Y., Tsuchiya M.

Japanese Journal of Gastroenterology 87 （ 8 ） 1701 - 1705 1990年08月

ISSN  0446-6586

Release of the serum immunosuppressive factor by monocytes in patients with crohn's disease

Watanabe M., Hibi T., Iwao Y., Kanai T., Ohara M., Fujisawa Ogata T. H., Watanabe N., Asakura H., Aiso S., Tsuru S., Tsuchiya M.

Journal of Clinical and Laboratory Immunology 29 （ 4 ） 161 - 166 1989年

ISSN  0141-2760

　概要を見る

<p>We investigated the in vitro immunosuppressive effect of the sera and the culture supernatants of the peripheral blood mononuclear cells obtained from patients with Crohn's disease. Sera from Crohn's disease markedly suppressed the proliferative response of mouse spleen cells, compared with sera from ulcerative colitis, intestinal tuberculosis and normal controls. The culture supernatants of the adherent mononuclear cells from Crohn's disease showed a remarkable suppressive effect (96±1%), while the culture supernatants of the nonadherent mononuclear cells ha no suppressive activity (-10±16%). The culture supernatants of the adherent and non-adherent mononuclear cells from normal controls had no suppressive activity. The fractionization of the culture supernatants of the adherent cells from the Crohn's disease patients demonstrated that the fractions with high suppressive activity had similar or identical biochemical properties to the serum immunosuppressive fractions which has been reported previously. These results indicate that monocytes may release the serum immunosuppressive factor in Crohn's disease. This factor may contribute to the depression of the immune reactivity in the mucosal lesion and to the persistence of the stimulation of abnormal immune response in the intestinal wall.</p>