論文 - 内田　明花

The orientation of a decellularized uterine scaffold determines the tissue topology and architecture of the regenerated uterus in rats

Miki F., Maruyama T., Miyazaki K., Takao T., Yoshimasa Y., Katakura S., Hihara H., Uchida S., Masuda H., Uchida H., Nagai T., Shibata S., Tanaka M.

Biology of Reproduction （Biology of Reproduction）  100 （ 5 ） 1215 - 1227 2019年

ISSN  00063363

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© 2019 The Author(s) 2019. Published by Oxford University Press on behalf of Society for the Study of Reproduction. A decellularized uterine scaffold (DUS) prepared from rats permits recellularization and regeneration of uterine tissues when placed onto a partially excised uterus and supports pregnancy in a fashion comparable to the intact uterus. The underlying extracellular matrix (ECM) together with an acellular, perfusable vascular architecture preserved in DUS is thought to be responsible for appropriate regeneration of the uterus. To investigate this concept, we examined the effect of the orientation of the DUS-preserving ECM and the vascular architecture on uterine regeneration through placement of a DUS onto a partially defective uterine area in the reversed orientation such that the luminal face of the DUS was outside and the serosal face was inside. We characterized the tissue structure and function of the regenerated uterus, comparing the outcome to that when the DUS was placed in the correct orientation. Histological analysis revealed that aberrant structures including ectopic location of glands and an abnormal lining of smooth muscle layers were observed significantly more frequently in the reversed group than in the correct group (70% vs. 30%, P < 0.05). Despite the changes in tissue topology, the uteri regenerated with an incorrectly oriented DUS could achieve pregnancy in a way similar to uteri regenerated with a correctly oriented DUS. These results suggest that DUS-driven ECM orientation determines the regenerated uterus structure. Using DUS in the correct orientation is preferable when clinically applied. The disoriented DUS may deteriorate the tissue topology leading to structural disease of the uterus even though the fertility potential is not immediately affected.

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CD34 and CD49f Double-Positive and Lineage Marker-Negative Cells Isolated from Human Myometrium Exhibit Stem Cell-Like Properties Involved in Pregnancy-Induced Uterine Remodeling

Ono M, Kajitani T, Uchida H, Arase T, Oda H, Uchida S, Ota K, Nagashima T, Masuda H, Miyazaki K, Asada H, Hida N, Morikawa S, Ito M, Bulun SE, Okano H, Matsuzaki Y, Yoshimura Y, Maruyama T.

Biol Reprod 93 （ 2 ） 37 2015年06月

研究論文（学術雑誌）, 共著, 査読有り

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Serum estradiol level during withdrawal bleeding as a predictive factor for intermittent ovarian function in women with primary ovarian insufficiency

Miyazaki K, Miki F, Uchida S, Masuda H, Uchida H, Maruyama T.

Endocr J 62 （ 1 ） 93 - 99 2014年10月

研究論文（学術雑誌）, 共著, 査読有り

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Molecular analysis of a mutated FSH receptor detected in a patient with spontaneous ovarian hyperstimulation syndrome.

Uchida S.Uchida S, Uchida H, Maruyama T, Kajitani T, Oda H, Miyazaki K, Kagami M, Yoshimura Y.

PLoS One. 8 （ 9 ） e75478 2013年09月

研究論文（学術雑誌）, 査読有り

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Possible involvement of nerve growth factor in dysmenorrhea and dyspareunia associated with endometriosis

Kajitani T, Maruyama T, Asada H, Uchida H, Oda H, Uchida S, Miyazaki K, Arase T, Ono M, Yoshimura Y.

Endocr J 60 （ 10 ） 1155 - 1164 2013年07月

研究論文（学術雑誌）, 共著, 査読有り

Stem cell-like differentiation potentials of endometrial side population cells as revealed by a newly developed in vivo endometrial stem cell assay

Miyazaki K, Maruyama T, Masuda H, Yamasaki A, Uchida S, Oda H, Uchida H, Yoshimura Y.

PLoS One. 7 （ 12 ） e50749 2012年12月

研究論文（学術雑誌）, 共著, 査読有り

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Studies using an in vitro model show evidence of involvement of epithelial-mesenchymal transition of human endometrial epithelial cells in human embryo implantation

Uchida H, Maruyama T, Nishikawa-Uchida S, Oda H, Miyazaki K, Yamasaki A, Yoshimura Y.

J Biol Chem. 287 （ 7 ） 4441 - 4450 2012年02月

研究論文（学術雑誌）, 共著, 査読有り

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Psychological adjustment and psychosocial stress among Japanese couples with a history of recurrent pregnancy loss

Kagami M, Maruyama T, Koizumi T, Miyazaki K, Nishikawa-Uchida S, Oda H, Uchida H, Fujisawa D, Ozawa N, Schmidt L, Yoshimura Y.

Hum Reprod. 27 （ 3 ） 787 - 794 2012年01月

研究論文（学術雑誌）, 共著, 査読有り

OCT4 expression in human uterine myometrial stem/progenitor cells

Ono, M., Kajitani, T., Uchida, H., Arase, T., Oda, H., Nishikawa-Uchida, S., Masuda, H., Nagashima, T., Yoshimura, Y. and Maruyama, T.

Hum Reprod. 25 （ 8 ） 2059 - 2067 2010年06月

研究論文（学術雑誌）, 共著, 査読有り

The UDP-glucose receptor P2RY14 triggers innate mucosal immunity in the female reproductive tract by inducing IL-8.

Arase, T., Uchida, H., Kajitani, T., Ono, M., Tamaki, K., Oda, H., Nishikawa, S., Kagami, M., Nagashima, T., Masuda, H., Asada, H., Yoshimura, Y., and Maruyama, T.

J Immunol. 182 （ 11 ） 7074 - 7084 2009年06月

研究論文（学術雑誌）, 共著, 査読有り

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Activation of SRC kinase and phosphorylation of STAT5 are required for decidual transformation of human endometrial stromal cells

Nagashima, T., Maruyama, T., Uchida, H., Kajitani, T., Arase, T., Ono, M., Oda, H., Kagami, M., Masuda, H., Nishikawa, S., Asada, H., and Yoshimura, Y.

Endocrinology. 149 （ 3 ） 1227 - 1234 2007年07月

研究論文（学術雑誌）, 共著, 査読有り

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