Nagao, Keisuke

写真a

Affiliation

School of Medicine, Department of Dermatology ( Shinanomachi )

Position

Professor
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Books 【 Display / hide 】

  • カラーアトラスDermoscopy

    永尾圭介，　 田中　勝/U>, 金原出版：　東京, 2003.11

    Scope: 192-193

  • カラーアトラスDermoscopy

    NAGAO KEISUKE, 金原出版, 2003

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Papers 【 Display / hide 】

  • Commensal-derived trehalose monocorynomycolate triggers γδ T cell-driven protective ocular barrier immunity

    Xu X., Rigas Y.E., Mattapallil M.J., Guo J., Sakamoto K., Nagao K., Nagarajan V., Bohrnsen E., Richards C., Gupta A., Gaud G., Love P.E., Jiang T., Zhang A., Xu B., Peng Z., Jittayasothorn Y., Carr M.A., Magone M.T., Brandes N.T., Shane J., Schwarz B., St. Leger A.J., Caspi R.R.

    Immunity 59 ( 2 ) 388 - 402.e5 2026.02

    Research paper (scientific journal), Accepted,  ISSN  10747613

    　View Summary

    Commensals shape host physiology through molecular crosstalk with host receptors. Identifying specific microbial factors that causally influence host immunity is key to understanding homeostasis at the host-microbe interface and advancing microbial-based therapeutics. Here, we identified trehalose monocorynomycolate (TMCM) from Corynebacterium mastitidis as a potent stimulator of interleukin 17 (IL-17) production by Vγ4 γδ T cells at the ocular surface. Mechanistically, TMCM-induced IL-17 responses depended on IL-1R and γδ T cell receptor (TCR) signaling, with TCR engagement further enhancing IL-1R1 expression on γδ T cells. Synthetic TMCM alone recapitulated the effect of Corynebacterium mastitidis in eliciting protective γδ T cell immunity at the ocular surface to prevent bacterial infection. Moreover, TMCM also promoted protective immunity in downstream eye-draining tissues and skin. These findings establish TMCM as a broadly applicable mediator of commensal-driven immune defense and highlight its therapeutic potential to strengthen IL-17-mediated protection at barrier sites.

  • Allogeneic hematopoietic cell transplantation for partial RAG deficiency in children and adults: Excellent outcomes with a reduced-intensity posttransplantation cyclophosphamide–based approach

    Dimitrova D., Bosticardo M., Delmonte O.M., Kenney H., An A., Pala F., Magro G., Myint-Hpu K., Kang E., Santangeli E., Angelova A., Vujkovic-Cvijin I., Schwarz B., Campos J., Chai A., Cusmano A., Flomerfelt F.A., Hyder M.A., Mangusan R., Rechache K., Sabina R., Telford W., Kong H.H., Nagao K., Agharahimi A., Bergerson J.R.E., Freeman A.F., Holland S.M., Ale H., El-Marsafy A., Pasic S., Verbsky J., Walter J., Kanakry C.G., Notarangelo L.D., Kanakry J.A.

    Journal of Allergy and Clinical Immunology  2026

    Research paper (scientific journal), Accepted,  ISSN  00916749

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    BackgroundPartial recombinase activating gene deficiency (pRD) leads to combined immunodeficiency with immune dysregulation. It can be cured by allogeneic hematopoietic cell transplantation (HCT), but optimal referral criteria and approaches remain to be defined.ObjectiveOur study evaluated low-toxicity approaches to HCT for pRD.MethodsThirteen children and adults with pRD received radiation-free, predominantly reduced-intensity conditioning (pentostatin/cyclophosphamide/busulfan) HCT with posttransplantation cyclophosphamide–based graft-versus-host disease (GVHD) prophylaxis at median (range) age 20 (4-46) years.ResultsWith median 2.6 years’ follow-up, overall survival for the entire cohort was estimated at 92% and 83% at 1 and 2 years and 100% and 90% for reduced-intensity conditioning recipients (n = 12), with 2 deaths attributed to sepsis. Reversal of clinical manifestations was associated with immune reconstitution, with minimal de novo autoimmunity, 15% 1-year cumulative incidence of grade III-IV acute GVHD, and no chronic GVHD. Vα7.2-positive T-cell proportion increased rapidly after HCT, while mucosa-associated invariant T-cell reconstitution lagged. Dysreactive CD19hiCD21lo and 9G4+ B cells decreased after HCT, along with clinically relevant autoantibodies. However, baseline elevated anti–type I interferon antibodies, potentially predisposing to severe viral infections, decreased slowly, although neutralizing activity was reduced at last follow-up. Outcomes did not differ by donor carrier status or HLA matching. Bronchiectasis exacerbations incurred rehospitalizations in long-term follow-up of patients who entered HCT with irreversible lung disease.ConclusionReduced-intensity conditioning HCT with posttransplantation cyclophosphamide–based GVHD prophylaxis is safe and effectively reverses immune dysfunction in patients with pRD.

  • It Mite Not Be Graft-Versus-Host Disease: Frequency and Clinical Features of Demodex Folliculitis After Allogeneic Hematopoietic Cell Transplantation

    Xue E., Cusmano A., Hyder M.A., Dimitrova D., Sabina R., Rechache K., Allbritton J., Huang E., Brownell I., Castelo-Soccio L., Nagao K., Kong H.H., Karacki K., Cousineau-Krieger C., Magone M.T., Cowen E.W., Kanakry J.A., Kanakry C.G.

    Transplantation and Cellular Therapy  2026

    Research paper (scientific journal), Accepted,  ISSN  26666375

  • Cellular stress-induced eccrine gland dysfunction as a potential mechanism in acquired idiopathic generalized anhidrosis

    Kageyama R., Sakamoto K., Nakamizo S., Kabashima K., Nakagawa M., Nagao K., Honda T.

    Journal of Investigative Dermatology  2026

    Research paper (scientific journal), Accepted,  ISSN  0022202X

    　View Summary

    Acquired idiopathic generalized anhidrosis (AIGA) is a rare disorder characterized by systemic anhidrosis or hypohidrosis of unknown etiology. Although autoimmune responses targeting eccrine glands and/or ducts have been proposed as a potential mechanism, the pathophysiology remains largely unclear. Corticosteroid pulse therapy is widely used for treatment, yet its mechanism of action is not fully understood. To elucidate the underlying mechanisms, we analyzed skin lesions from patients with AIGA before and after corticosteroid pulse therapy through histological and single-cell RNA-sequencing analyses. AIGA cases were histologically classified into pauci-inflammatory, mild inflammatory, and severe inflammatory types on the basis of lymphocytic infiltration around the eccrine unit. Corticosteroid pulse therapy improved sweating function across all groups, including pauci-inflammatory cases with little immune infiltration. Single-cell RNA sequencing of pauci-inflammatory AIGA skin revealed significant upregulation of eccrine marker genes such as MUCL1 and DCD alongside downregulation of cellular stress response pathways associated with unfolded protein responses after therapy. Immunohistochemistry confirmed increased expression of eccrine markers and reduced cellular stress markers, including advanced glycation end products and 4-hydroxynonenal, in eccrine glands after treatment. These findings suggest that unfolded protein response–associated cellular stress–mediated eccrine dysfunction contributes to AIGA pathogenesis, providing a basis for exploring cellular stress modulation as a potential therapeutic approach.

  • Upregulated natriuretic peptide B expression as a hallmark of chronic itch

    Tseng P.Y., Liew H.L., Ringkamp M., Nagao K., Hoon M.A.

    Pain 166 ( 12 ) 2818 - 2830 2025.12

    Research paper (scientific journal), Accepted,  ISSN  03043959

    　View Summary

    Abstract – Chronic itch can arise from a variety of etiologies, ranging from dermatological conditions like eczema and psoriasis to systemic diseases such as liver disease and kidney failure. However, it remains unclear whether there are common molecular features associated with chronic itch, and whether these features are selective for chronic itch compared to chronic pain. To identify potential genes or molecular characteristics that are specifically associated with chronic itch, we examined transcriptomic data from sensory neurons collected from 3 mouse models of chronic itch and a monkey model of contact dermatitis. We compared these data to transcriptomic data from 3 mouse models of pain and clinical data from patients with neuropathic pain. Our analyses revealed that the upregulation of Nppb expression in sensory neurons is consistently associated with models of itch, but not with models of pain. Further, our cellular characterization showed that the increased expression of Nppb arises from increased cell-autonomous expression rather than the recruitment of Nppb expression in other classes of sensory neurons. Given that Nppb is a well-established itch neurotransmitter, our findings suggest that the increased expression of Nppb in sensory neurons may contribute to chronic itch. In addition, based on our results, we propose that Nppb could serve as a conserved biomarker for chronic itch.

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Reviews, Commentaries, etc. 【 Display / hide 】

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Presentations 【 Display / hide 】

  • グロームス腫瘍の3例

    NISHIKAWA TAKEJI

    [Domestic presentation]  第786回日本皮膚科学会東京地方会 (東京) , 

    2003.11
    , 

    Oral presentation (general)

  • 皮膚ムコール症3例より分離されたRhizopus属の走査電顕所見

    ISHIKO AKIRA

    [Domestic presentation]  第30回日本電顕皮膚生物学会 (金沢) , 

    2003.09
    , 

    Oral presentation (general)

  • Mucinous carcinoma of the skinの1例

    TANAKA MASARU

    [Domestic presentation]  第782回日本皮膚科学会東京地方会 (東京) , 

    2003.06
    , 

    Oral presentation (general)

  • A case of pemphigus foliaceus indused by D-penicillamine

    Nagao Keisuke, Tanikawa Akiko, Amagai Masayuki

    [International presentation]  Japan-China Joint-Meeting of Dermatology, 

    2002.12
    , 

    Oral presentation (general)

  • タクロリムス軟膏で加療した膿疱性乾癬の1例

    Nagao Keisuke, Ishikou Akira, Tanikawa Akiko, Amagai Masayuki

    [Domestic presentation]  第17回日本乾癬学会学術大会, 

    2002.10
    , 

    Oral presentation (general)

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