論文 - チッテリオ，　ダニエル

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チッテリオ　 ダニエル

Analytical Sciences （Japan Society for Analytical Chemistry）  34 （ 1 ） 5 - 6 2018年01月

研究論文（学術雑誌）, 共著,  ISSN  0910-6340

Wax-assisted one-step enzyme-linked immunosorbent assay on lateral flow test devices

チッテリオ　 ダニエル

Analytical Sciences （Analytical Sciences）  34 （ 1 ） 51 - 56 2018年01月

研究論文（学術雑誌）, 共著, 査読有り,  ISSN  0910-6340

　概要を見る

© The Japan Society for Analytical Chemistry, 2018. Lateral flow tests (LFTs) are widely used analytical tools characterized by portability, operator simplicity and short analysis times. A remaining challenge is their limited analytical sensitivity, which in classical immunoassay formats is overcome by enzyme-linked immunosorbent assay (ELISA) formats. The implementation of ELISA to an LFT format however, is hampered by the complexity of the procedure requiring the enzyme substrate addition after sample addition. In this work, a simple method for automation of this procedure without user interference is presented. Originally used sample pads of LFTs have been replaced by hydrophobic wax-modified filter paper-based sample pads to realize a delayed flow a pre-deposited colorimetric ELISA substrate without other alterations to the classical lateral-flow immunoassay format. The performance of the system has been characterized by visualizing flow behavior and final proof-of-concept is provided by a model mouse IgG assay, achieving a limit of detection of 15.8 ng mL -1 from just a single application of the sample solution.

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"dip-and-read" paper-based analytical devices using distance-based detection with color screening

Yamada K., Citterio D., Henry C.

Lab on a Chip （Lab on a Chip）  18 （ 10 ） 1485 - 1493 2018年

ISSN  14730197

　概要を見る

© 2018 The Royal Society of Chemistry. An improved paper-based analytical device (PAD) using color screening to enhance device performance is described. Current detection methods for PADs relying on the distance-based signalling motif can be slow due to the assay time being limited by capillary flow rates that wick fluid through the detection zone. For traditional distance-based detection motifs, analysis can take up to 45 min for a channel length of 5 cm. By using a color screening method, quantification with a distance-based PAD can be achieved in minutes through a "dip-and-read" approach. A colorimetric indicator line deposited onto a paper substrate using inkjet-printing undergoes a concentration-dependent colorimetric response for a given analyte. This color intensity-based response has been converted to a distance-based signal by overlaying a color filter with a continuous color intensity gradient matching the color of the developed indicator line. As a proof-of-concept, Ni quantification in welding fume was performed as a model assay. The results of multiple independent user testing gave mean absolute percentage error and average relative standard deviations of 10.5% and 11.2% respectively, which were an improvement over analysis based on simple visual color comparison with a read guide (12.2%, 14.9%). In addition to the analytical performance comparison, an interference study and a shelf life investigation were performed to further demonstrate practical utility. The developed system demonstrates an alternative detection approach for distance-based PADs enabling fast (∼10 min), quantitative, and straightforward assays.

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Thread-based analytical device for antibody detection in whole blood by using bioluminescent sensor proteins

Tomimuro K., Tenda K., Hiruta Y., Merkx M., Citterio D.

22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 （22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018）  2   1018 - 1021 2018年

　概要を見る

Bioluminescent protein integrating microfluidic thread-based analytical devices (µTADs) for point-of-care testing (POCT) of antibodies in whole blood have been developed. Devices consist of a blood separation membrane and a plastic film layer with sewn-in cotton thread, in which proteins and luciferin for signal generation are pre-deposited. Colorimetry-based response curves for three model antibodies spiked into porcine whole blood have been obtained. Stable signals from 6-18 min after sample application and no cross-talk when assaying multiple different antibodies are observed. The µTADs offer quantitative simultaneous detection of multiple antibodies in a small volume of whole blood (8 µL).

Text-displaying competitive immunochromatographic strips enabling naked-eye semi-quantitative analysis

Misawa K., Yamamoto T., Watanabe D., Hiruta Y., Yamazaki H., Citterio D.

22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 （22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018）  2   1022 - 1025 2018年

　概要を見る

Competitive lateral flow immunochromatographic assays (LFIAs) enabling naked-eye semi-quantitative result readout in the form of “text” have been developed. User operation is limited to sample application, followed by direct reading of assay results written in text representing the target concentration. LFIA strips enabled the detection of 8-hydroxy-2’-deoxyguanosine (8-OHdG) at concentrations down to 5 ng/mL, which is comparable to existing immunochromatography using external readout equipment. The system is readily adaptable to other analytical targets and allows for high flexibility in text design and detectable analyte concentrations. The simple assay handling and intuitive data interpretation offer great potential for point-of-care testing applications.

Fully Inkjet-Printed Paper-Based Potentiometric Ion-Sensing Devices

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Analytical Chemistry （The American Chemical Society）  89 （ 19 ） 10608 - 10616 2017年10月

研究論文（学術雑誌）, 共著, 査読有り,  ISSN  0003-2700

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Fabrication of a New Lineage of Artificial Luciferases from Natural Luciferase Pools

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ACS Combinatorial Science （The American Chemical Society）  19 （ 9 ） 594 - 599 2017年09月

研究論文（学術雑誌）, 共著, 査読有り,  ISSN  2156-8952

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Text-Displaying Colorimetric Paper-Based Analytical Device

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ACS Sensors （The American Chemical Society）  2 （ 8 ） 1247 - 1254 2017年08月

研究論文（学術雑誌）, 共著, 査読有り,  ISSN  2379-3694

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"Drop-Slip" Bulk Sample Flow on Fully Inkjet-Printed Microfluidic Paper-Based Analytical Device

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Sensors and Actuators B - Chemical （Elsevier）  244   1129 - 1137 2017年06月

研究論文（学術雑誌）, 共著, 査読有り,  ISSN  0925-4005

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Click Reaction Based on the Biosynthesis of Firefly Luciferin

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Chemistry Letters （The Chemical Society of Japan）  46 （ 5 ） 753 - 755 2017年05月

研究論文（学術雑誌）, 共著, 査読有り,  ISSN  0366-7022

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Luciferase-Specific Coelenterazine Analogues for Optical Contamination-Free Bioassays

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Scientific Reports （Springer Nature）  7   908 2017年04月

研究論文（学術雑誌）, 共著, 査読有り,  ISSN  2045-2322

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Paper-Based Analytical Device for Zinc Ion Quantification in Water Samples with Power-Free Analyte Concentration

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Micromachines （MDPI）  8 （ 4 ） 127 2017年04月

研究論文（学術雑誌）, 共著, 査読有り,  ISSN  2072-666X

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Spirolactam Capped Cyanine Dyes for Designing NIR Probes to Target Multiple Metal Ions

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RSC Advances （The Royal Society of Chemistry）  7 （ 40 ） 24970 - 24980 2017年

研究論文（学術雑誌）, 共著, 査読有り,  ISSN  2046-2069

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Real-time Monitoring and Detection of Primer Generation-Rolling Circle Amplification of DNA Using an Ethidium Ion-selective Electrode

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Analytical Sciences （Japan Society for Analytical Chemistry）  32 （ 5 ） 505 - 510 2016年05月

研究論文（学術雑誌）, 共著, 査読有り,  ISSN  0910-6340

High-Resolution Microfluidic Paper-Based Analytical Devices for Sub-Microliter Sample Analysis

チッテリオ　 ダニエル

Micromachines （MDPI）  7 （ 5 ） 80 2016年05月

研究論文（学術雑誌）, 共著, 査読有り,  ISSN  2072-666X

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Genetically Encoded Molecular Tension Probe for Tracing Protein - Protein Interactions in Mammalian Cells

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BIOCONJUGATE CHEMISTRY 27 （ 2 ） 354 - 362 2016年02月

研究論文（学術雑誌）, 共著,  ISSN  1043-1802

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Distance-Based Tear Lactoferrin Assay on Microfluidic Paper Device Using Interfacial Interactions on Surface-Modified Cellulose

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ACS APPLIED MATERIALS & INTERFACES 7 （ 44 ） 24864 - 24875 2015年11月

研究論文（学術雑誌）, 共著,  ISSN  1944-8244

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A Fluorous Biphasic Solvent Extraction System for Lanthanides with a Fluorophilic beta-Diketone Type Extractant

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ANALYTICAL SCIENCES 31 （ 9 ） 923 - 928 2015年09月

研究論文（学術雑誌）, 共著,  ISSN  0910-6340

Near IR Emitting Red-Shifting Ratiometric Fluorophores Based on Borondipyrromethene

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ORGANIC LETTERS 17 （ 12 ） 3022 - 3025 2015年06月

研究論文（学術雑誌）, 共著,  ISSN  1523-7060

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Papierbasierte tintenstrahlgedruckte Mikrofluidiksysteme für die Analytik

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Angewandte Chemie （Wiley）  127 （ 18 ） 5384 - 5401 2015年04月

研究論文（学術雑誌）, 共著, 査読有り,  ISSN  1521-3757

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