Imoto, Masaya

写真a

Affiliation

Faculty of Science and Technology （Mita）

Position

Professor Emeritus
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Career 【 Display / hide 】

  • 1980.04
    -
    1989.03

    キリンビール株式会社 ,勤務

  • 1989.04
    -
    1991.03

    慶應義塾大学理工学部 ,助手

  • 1991.04
    -
    1996.03

    慶應義塾大学理工学部 ,専任講師

  • 1996.04
    -
    2002.03

    慶應義塾大学理工学部 ,助教授

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Academic Background 【 Display / hide 】

  • 1978.03

    Yamaguchi University, Faculty of Agriculture, 農芸化学科

    University, Graduated

  • 1980.03

    Yamaguchi University, Graduate School, Division of Agriculture, 農芸化学専攻

    Graduate School, Completed, Master's course

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Academic Degrees 【 Display / hide 】

  • 農学　, The University of Tokyo, 1988.02

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Research Areas 【 Display / hide 】

  • Nanotechnology/Materials / Chemical biology

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Books 【 Display / hide 】

  • Apoptosis and autophagy

    Tashiro E., Kitagawa M., Imoto M., Bioprobes: Biochemical Tools for Investigating Cell Function: Second Edition, 2017.05

    　View Summary

    Apoptosis and autophagy are highly coordinated mechanisms to maintain cellular homeostasis against various intrinsic and/or extrinsic stresses in eukaryotic cells. Since the early 1990s, our knowledge of the molecular mechanisms and the physiological functions of apoptosis have increased substantially. Dysfunction of apoptosis leads to various diseases, including cancers and degenerative diseases. Therefore, small molecules that induce apoptosis or synthetic lethality with mutated genes have been examined for the development of cancer-specific apoptosis-inducing agents; conversely, small molecules that suppress apoptosis have been identified for protective agents against neuronal cell death. On the other hand, autophagy is an evolutionarily conserved pathway involved in the degradation of intracellular components and is critical for the maintenance of cellular homeostasis. The mechanisms and functions of autophagy have been revealed by genetic studies in yeast, which identified a series of autophagy-related genes. Many small molecules that have been discovered to induce or inhibit autophagy also provide insight into the mechanisms and functions of the autophagic process. In this chapter, we introduce several small molecules identified by synthetic lethality screening, apoptosis inhibitors, and autophagy modulators.

  • Chemical Biology of Cell Motility Inhibitors

    Kawamura T., Kitagawa M., Imoto M., Protein Targeting with Small Molecules: Chemical Biology Techniques and Applications, 2009.08

  • バイオの窓「大学における物取り研究」

    バイオサイエンスとインダストリー59: 43 (2001), 2001.03

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Papers 【 Display / hide 】

  • Isolation of Caldorazole, a Thiazole-Containing Polyketide with Selective Cytotoxicity under Glucose-Restricted Conditions

    Osamu Ohno, Arihiro Iwasaki, Kyouhei Same, Chihiro Kudo, Erika Aida, Kazuya Sugiura, Shimpei Sumimoto, Toshiaki Teruya, Etsu Tashiro, Siro Simizu, Kenji Matsuno, Masaya Imoto, Kiyotake Suenaga

    ORGANIC LETTERS （AMER CHEMICAL SOC)   2022.06

    ISSN  1523-7060

    　View Summary

    Caldorazole (1) was isolated from the marine cyanobacterium Caldora sp. collected on Ishigaki Island, Okinawa, Japan. Its structure was determined to be a new polyketide that contained two thiazole rings and an O-methylenolpyruvamide moiety. Caldorazole (1) showed strong cytotoxicity toward tumor cells that had been seeded at a high density. Cell death induced by 1 in HeLa and A431 cells was also observed only in the presence of the glycolysis blocker 2-deoxy-D-glucose (2DG). Co-treatment with 1 and 2DG remarkably decreased ATP levels in these cells. Furthermore, 1 selectively inhibited complex I in the mitochondrial respiratory chain. Thus, 1 was demonstrated to exert cytotoxicity toward human tumor cells by blocking mitochondrial respiration.

  • Autophagy is Required for the Maintenance of NAD

    Tetsushi Kataura, Lucia Sedlackova, Elsje G. Otten, David Shapira, Filippo Scialo, Rhoda Stefanatos, Kei-ichi Ishikawa, George Kelly, Elena Seranova, Congxin Sun, Dorothea Maetzel, Niall Kenneth, Sergey Trushin, Tong Zhang, Eugenia Trushina, Charles C. Bascom, Ryan Tasseff, Robert J. Isfort, John E. Oblong, Satomi Miwa, Michael Lazarou, Rudolf Jaenisch, Masaya Imoto, Shinji Saiki, Manolis Papamichos-Chronakis, Oliver D.K. Maddocks, Alberto Sanz, Sovan Sarkar, Viktor Korolchuk

    SSRN Electronic Journal （Elsevier BV)   2022

  • Involvement of miR-3180-3p and miR-4632-5p in palmitic acid-induced insulin resistance

    Tashiro Etsu, Nagasawa Yumi, Itoh Susumu, Imoto Masaya

    MOLECULAR AND CELLULAR ENDOCRINOLOGY 534 2021.08

    ISSN  0303-7207

  • Androgen receptor antagonists produced by Streptomyces overcome resistance to enzalutamide

    Masaya Imoto, Takahiro Fujimaki, Shun Saito, Etsu Tashiro

    The Journal of Antibiotics （Springer Science and Business Media LLC)  74 （ 10 ） 706 - 716 2021.07

    ISSN  0021-8820

  • Cyclopeptides from the Mushroom Pathogen Fungus Cladobotryum varium

    Tao Zhou, Misaki Katsuragawa, Tian Xing, Keisuke Fukaya, Toru Okuda, Toshiyuki Tokiwa, Etsu Tashiro, Masaya Imoto, Naoya Oku, Daisuke Urabe, Yasuhiro Igarashi

    JOURNAL OF NATURAL PRODUCTS （AMER CHEMICAL SOC)  84 （ 2 ） 327 - 338 2021.02

    ISSN  0163-3864

    　View Summary

    Three new cyclopeptides with serial Phe residues were identified with the aid of HPLC-DAD analysis, from the culture broth of Cladobotryum varium, a fungal pathogen causing mushroom cobweb disease. Cladoamides A (1) and B (2) have two consecutive N-methylphenylalanine units in the destruxin class cyclic depsipentapeptide framework, while cladoamide C (3) has a three consecutive Phe motif in a cyclopentapeptide structure. Of these three cyclopeptides, 1 showed potent autophagy-inducing activity at 10 mu g/mL, comparable to a positive control, rapamycin. For the determination of the absolute configurations of the Ile residues in 1 and 3, new conditions for separating Ile and alto-Ile, using a pentafluorophenyl-bonded solid phase and methanolic solvent, were established within the analytical scheme of the advanced Marfey's method, thus offering a convenient alternative to the C-3 Marfey's method, which requires elution with a threesolvent mixture. The sequence of two D-Phe and one L-Phe in 3 was determined through NMR chemical shift prediction by DFT-based calculations and chemical synthesis, which demonstrated the significance of noncovalent interactions in the accurate calculation of stable conformers for peptides with multiple aromatic rings.

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Papers, etc., Registered in KOARA 【 Display / hide 】

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Reviews, Commentaries, etc. 【 Display / hide 】

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Research Projects of Competitive Funds, etc. 【 Display / hide 】

  • Chemical Biology for Parkinson's disease

    2018.04
    -
    2021.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Principal investigator

  • Chemical Biological studies for autophagy

    2015.04
    -
    2018.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, IMOTO MASAYA, Tashiro Etsu, Grant-in-Aid for Scientific Research (B), Principal investigator

    　View Summary

    We constructed GFP-LC3-RFP-expressing cells, and using the cells, we analyzed the diversity and consistency of regulatory signaling in autophagy. The effects of more than 200 small molecular compounds were assessed quantitatively by autophagy regulatory system in cancer cells and neuronal cells. Hierarchical clustering was performed on the subsequent autophagy inhibition profile of the compounds in each cell type. The result was that hierarchical clustering accurately classified the compounds according to their targets. And we distinguished between common and cell type-specific signals responsible for autophagy. SO286, which increased autophagy flux, inhibited the Aggresome formation. SO286 was found to bind SOBP, and by knockdown of SOBP, SO286 failed to inhibit Aggresome formation. This result indicated that SO286 functioned SOBP-dependent manner. We searched for the compounds that induce autophagic cell death, and we isolated Cholest-5-ene-3b,7a-diol from microbial origin.

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Intellectual Property Rights, etc. 【 Display / hide 】

  • デフォスタチンの物質特許

    Date issued：   1996

    Patent

  • 新規チロシンキナーゼ阻害剤の物質特許

    Date issued：   1985

    Patent

  • イノスタマイシンの物質特許

    Date issued：   1989

    Patent

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Awards 【 Display / hide 】

  • 住木・梅沢記念賞受賞

    2000.11, 日本抗生物質学術協議会

  • 住木・梅沢記念賞

    2000, 日本感染症医薬品協会（旧日本抗生物質学術協議会）, 微生物由来細胞内情報伝達系に作用する物質の探索研究

  • 日本農芸化学会 奨励賞

    1995.03, 日本農芸化学会, 細胞内情報伝達系阻害剤の発見により受賞した。

  • 日本農芸化学会奨励賞受賞

    1995.03, 日本農芸化学会

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Courses Taught 【 Display / hide 】

  • ADVANCED LABORATORY COURSE IN BIOSCIENCES AND INFORMATICS A

    2019

  • ADVANCED LABORATORY COURSE IN BIOSCIENCES AND INFORMATICS B

    2019

  • ADVANCED SCIENCES FOR DRUG DISCOVERY

    2019

  • BACHELOR'S THESIS

    2019

  • BASIC LABORATORY COURSE IN BIOSCIENCES

    2019

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Social Activities 【 Display / hide 】

  • バイオサイエンスとインダストリー誌

    1998.04
    -
    Present

  • 癌分子標的治療研究会

    1997
    -
    Present
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Committee Experiences 【 Display / hide 】

  • 1998.04
    -
    Present

    トピックス委員, バイオサイエンスとインダストリー誌

  • 1997
    -
    Present

    会員, 癌分子標的治療研究会

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