花屋　賢悟 （ハナヤ　ケンゴ）

Hanaya, Kengo

写真a

所属（所属キャンパス）

薬学部 薬科学科 有機薬化学講座 （芝共立）

職名

専任講師
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  • 博士（薬学）, 東京理科大学, 課程, 2012年03月

    Design and Synthesis of Biomedical Tools and Metalloprotease Inhibitors Based on Chemical Properties of 8-Quinolinol and Their Sulfonates

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  • Structural Elucidation of Nucleophilic Compounds through Synergistic Coordination and Hydrogen Bonding in a Metal–Organic Framework

    Nakagawa T., Wada Y., Chan B., Baba T., Hanaya K., Koseki Y., Asano R., Aoki K., Usov P.M., Kawano M.

    Journal of the American Chemical Society 147 （ 32 ） 29013 - 29025 2025年08月

    ISSN  00027863

    　概要を見る

    This study presents the development of a novel metal–organic framework (MOF) denoted as APF-80, suitable for the structural analysis of nucleophilic compounds that have traditionally been challenging to analyze using the crystalline sponge method. It was synthesized using a new mixed-substituent hexaazaphenalene ligand (344-TPHAP) featuring both 3-pyridyl and 4-pyridyl groups. This framework demonstrated remarkable stability toward nucleophilic molecules, which could be captured inside the pores through a synergistic combination of coordination and hydrogen-bonding interactions. APF-80 was successfully applied to determine the structures of 12 bioactive molecules, including naturally occurring alkaloids and pharmaceutical compounds. The host–guest interaction modes observed inside the resulting structures were classified into five distinct types. Binding energy calculations revealed that the guest site energies positively correlated with the crystallographic occupancies and the corresponding interaction types. This multimodal capture mechanism enabled the precise structural analysis of nucleophilic compounds under mild conditions, expanding the scope of molecules that can be analyzed by the crystalline sponge method.

  • One-Step Maleimide-Based Dual Functionalization of Protein N-Termini

    Hanaya K., Taguchi K., Wada Y., Kawano M.

    Angewandte Chemie - International Edition （Wiley）  64 （ 5 ） e202417134 2024年11月

    研究論文（学術雑誌）, 共著, 筆頭著者, 責任著者, 査読有り,  ISSN  14337851

    　概要を見る

    Maleimide derivatives are privileged reagents for chemically modifying proteins through the Michael addition reaction with cysteine due to their selectivity, operational simplicity, and commercial availability. However, since accessible free cysteine is rarely found in natural proteins, it is highly desirable to find alternative targets to enable direct bioconjugation of proteins with maleimides. In this study, we have developed an operationally simple and straightforward method for the N-terminal modification of proteins without the need for mutagenesis via a copper(II)-mediated [3+2] cycloaddition reaction with maleimides and 2-pyridinecarboxaldehyde (2-PCA) derivatives under non-denaturing conditions at pH 6 and 37 °C in aqueous media. Our method utilizes commercially available maleimides to attach diverse functionalities to various N-terminal amino acids. We demonstrate the preparation of a ternary protein complex cross-linked at the N-termini and dually modified trastuzumab equipped with monomethyl auristatin E (MMAE), a cytotoxic agent, and a Cy5 fluorophore (MMAE-Cy5-trastuzumab). MMAE-Cy5-trastuzumab retained human epidermal growth factor receptor 2 (HER2) recognition activity and exerted cytotoxicity against HER2-positive cells. Furthermore, MMAE-Cy5-trastuzumab allowed successful visualization of HER2-positive cancer cells in mouse tumors. This straightforward method will expand the accessibility of protein conjugates with well-defined structures in a wide range of research fields.

  • Dual delivery of carbon monoxide and doxorubicin using haemoglobin-albumin cluster: proof of concept for well-tolerated cancer therapy

    Ito C., Taguchi K., Yamada T., Hanaya K., Enoki Y., Sugai T., Komatsu T., Matsumoto K.

    Journal of Materials Chemistry B 12 （ 23 ） 5600 - 5608 2024年05月

    ISSN  2050750X

    　概要を見る

    A serious concern of doxorubicin (DOX) therapy is that it causes severe adverse effects, particularly cardiotoxicity. Carbon monoxide (CO) possesses powerful cytoprotective effects against drug-induced organ injury and is expected to ameliorate DOX-induced cardiotoxicity. In this study, a dual carrier of DOX and CO (CO-HemoAct-DOX) was fabricated based on a haemoglobin-albumin cluster (HemoAct), which is a protein cluster with a haemoglobin core structure wrapped by serum albumin. CO-HemoAct-DOX was synthesised by binding CO to a haemoglobin core and covalently conjugating (6-maleimidocaproyl)hydrazone derivative of DOX to an albumin shell. The average DOX/cluster ratio was about 2.6. In the in vitro cytotoxicity assay against cancer cells, the anti-tumour activity of CO-HemoAct-DOX was 10-fold lower than that of DOX in a 2D-cultured model, whereas CO-HemoAct-DOX suppressed the growth of tumour spheroids to the same extent as DOX in the 3D-cultured model. In colon-26 tumour-bearing mice, CO-HemoAct-DOX achieved DOX delivery to the tumour site and alleviated tumour growth more effectively than DOX. Furthermore, CO-HemoAct attenuated DOX-induced cardiomyocyte atrophy in H9c2 cells and elevated the levels of cardiac biomarkers in mice exposed to DOX. These results suggest that the dual delivery of CO and DOX using HemoAct is a promising strategy as an anti-tumour agent to realise well-tolerated cancer therapy with minimal cardiotoxicity.

  • Synthesis and property of 2,2-difluoro-1,4-diazaborole

    Nakahara M., Wada Y., Kawano M., Hanaya K., Sugai T., Higashibayashi S.

    Chemistry Letters 53 （ 5 ）  2024年05月

    ISSN  03667022

    　概要を見る

    The synthesis of 2,2-difluoro-1,4-diazaboroles was achieved through the condensation of aliphatic acyltrifluoroborates and amino-N-heteroarenes under acidic conditions. Various N-heteroarenes, including pyridine, pyrimidine, pyridazine, thiazole, and isoquinoline, were successfully employed in the reaction. Among the synthesized 2,2-difluoro-1,4-diazaboroles, the one with an isoquinoline structure exhibited the most intense light blue emission.

  • Utilization of Lipase-Catalyzed Acetylation and Deacetylation in the Synthesis of a Natural Product with a Hydroquinone Skeleton

    Sugai T., Honda K., Hashimoto R., Hanaya K., Higashibayashi S.

    Asian Journal of Organic Chemistry 13 （ 12 ）  2024年

    　概要を見る

    Elaborated conditions for lipase-catalyzed preferential monoacetylation enabled the large-scale preparation of hydroquinone monoacetate. This product was then applied to the synthesis of tournefolin B, a highly oxygenated prenylated hydroquinone of plant origin (Tournefortia sarmentosa Lam.). During the course of the synthetic transformations, lipase-catalyzed deacetylation under mild conditions was demonstrated.

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競争的研究費の研究課題 【 表示 ／ 非表示 】

  • 金属結合モチーフの構築を志向したポリペプチド化学修飾

    2024年04月
    -
    2027年03月

    花屋 賢悟, 基盤研究(C), 補助金,  研究代表者

  • 一時的配向基を用いた遷移金属触媒反応によるタンパク質ペプチド結合の化学修飾

    2021年04月
    -
    2024年03月

    文部科学省・日本学術振興会, 科学研究費助成事業, 花屋 賢悟, 基盤研究(C), 補助金,  研究代表者

  • ボロン酸を用いるクロスカップリング反応を基盤としたタンパク質化学修飾法の開発

    2019年04月
    -
    2021年03月

    文部科学省・日本学術振興会, 科学研究費助成事業, 花屋　賢悟, 若手研究, 補助金,  研究代表者

  • スルホン酸エステルを母核とした刺激応答性リンカーの開発とプロドラッグへの応用

    2016年04月
    -
    2019年03月

    文部科学省・日本学術振興会, 科学研究費補助金(文部科学省・日本学術振興会）, 花屋 賢悟, 補助金,  未設定

  • 刺激応答性リンカーの創製に向けたアミノスルホン酸エステルの分子内環化反応の開発

    2016年04月
    -
    2017年03月

    公益財団法人　日本科学協会, 笹川科学研究助成, 花屋 賢悟, 補助金,  研究代表者

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担当授業科目 【 表示 ／ 非表示 】

  • 課題研究（薬化学）

    2025年度

  • 演習（薬化学）

    2025年度

  • 卒業研究１（薬学科）

    2025年度

  • 高度研究機器特別演習

    2025年度

  • 英語演習（薬学科）

    2025年度

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