Shinya Minori

写真a

Affiliation

Faculty of Business and Commerce ( Hiyoshi )

Position

Associate Professor
Scopus Paper Info  
Paper Count: 27 Citation Count: 1191 h-index: 15

Click to view the Scopus page. The data was downloaded from Scopus API in March 29, 2026, via http://api.elsevier.com and http://www.scopus.com .

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Papers 【 Display / hide 】

  • Establishment of a zebrafish inbred strain, M-AB, capable of regular breeding and genetic manipulation

    Sadamitsu K., Velilla F., Shinya M., Kashima M., Imai Y., Kawasaki T., Watai K., Hosaka M., Hirata H., Sakai N.

    Scientific Reports 14 ( 1 )  2024.12

    　View Summary

    Inbred strains of organisms are genetically highly uniform and thus useful for life science research. We have previously reported the ongoing generation of the zebrafish IM strain from the India (IND) strain through full sib-pair mating for 16 generations. However, the IM fish laid a small number of offspring and had a short lifespan, implying the need for discreet care in breeding. Here, we report the subsequent establishment of IM strain as well as the generation of a new inbred zebrafish strain, Mishima-AB (M-AB). M-AB was derived from the *AB strain by full sib-pair mating for over 20 generations, which fulfills the general criterion for the establishment of an inbred strain. In contrast to the IM case, maintenance of the M-AB strain by sib-pair mating required almost no special handling. Genome sequencing of IM individuals from the 47th generation and M-AB individuals from the 27th generation revealed that SNP-based genomic heterogeneity across whole-genome nucleotides was 0.008% and 0.011%, respectively. These percentages were much lower than those of the parental IND (0.197%) and *AB (0.086%) strains. These results indicate that the genomes of these inbred strains were highly homogenous. We also demonstrated the successful microinjection of antisense morpholinos, CRISPR/Cas9, and foreign genes into M-AB embryos at the 1-cell stage. Overall, we report the establishment of a zebrafish inbred strain, M-AB, which is capable of regular breeding and genetic manipulation. This strain will be useful for the analysis of genetically susceptible phenotypes such as behaviors, microbiome features and drug susceptibility.

  • High-speed system to generate congenic strains in medaka

    Shinya M., Kimura T., Naruse K.

    Genes and Genetic Systems 98 ( 5 ) 267 - 275 2023

    ISSN  13417568

    　View Summary

    The congenic strain, an inbred strain containing a small genomic region from another strain, is a powerful tool to assess the phenotypic effect of polymorphisms and/or mutations in the substituted genomic region. Recent substantial progress in the genetic studies of complex traits increases the necessity of congenic strains and, therefore, a quick breeding system for congenic strains has become increasingly important in model organisms such as mouse and medaka. Traditionally, more than ten generations are necessary to produce a congenic strain. In contrast, a quick method has been reported previously for the mouse, in which the use of genetic markers reduces the required number of backcross generations to about a half that of the traditional method, so that it would take around six generations to obtain a congenic strain. Here, we present an even quicker congenic production system, which takes only about four generations. The system can produce medaka congenic strains having part of the HNI-II (an inbred medaka strain derived from the northern Japanese population, Oryzias sakaizumii) genome in the HdrR-II1 (another inbred strain from the southern Japanese population, O. latipes) background. In this system, the availability of frozen sperm and genotype data of the BC<inf>1</inf> male population makes it possible to start marker-assisted congenic production after obtaining the BC<inf>2</inf> population. Our evaluation revealed that the system could work well to increase the percentage of recipient genome as expected, so that a congenic strain may be obtained in about one year.

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Papers, etc., Registered in KOARA 【 Display / hide 】

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Research Projects of Competitive Funds, etc. 【 Display / hide 】

  • Analysis to approach the mechanism for generating individual differences found in craniofacial morphology ~identification of the susceptible polymorphisms and genes~

    2022.04
    -
    2026.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, 基盤研究(C), Principal investigator

  • Variations in the medaka craniofacial morphology : searching for the responsible genes

    2014.04
    -
    2017.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Principal investigator

    　View Summary

    Craniofacial morphology is a complex but interesting trait with high variety within a species while maintaining the particular morphology of the species. Logical questions have been posed in order to address variations in cranial morphology, such as how these traits are inherited, which genes regulate the diversity, and how differences between individuals within a species are generated during developmental or life processes. To answer these questions, we had performed quantitative trait loci (QTL) analyses using medaka. QTL analysis suggested the relationships between L33 and chromosome (chr) 6, D29 and chr 15, D29 and chr 22, and V13 and chr 5. Narrowing analysis using congenic strains revealed that about 5 Mbp region of chr 6 affects to L33. On the other hand, D29 were failed to be confirmed the relationships with both chr 15 and 22. V13 was turned out to be too unstable to analyze further.

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Courses Taught 【 Display / hide 】

  • GENERAL EDUCATION SEMINAR (DB)

    2024

  • GENERAL EDUCATION SEMINAR (DA)

    2024

  • BIOLOGY 2

    2024

  • BIOLOGY 1

    2024

  • GENERAL EDUCATION SEMINAR (S)

    2023

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