新屋　みのり （ シンヤ　ミノリ ）

Shinya Minori

写真a

所属（所属キャンパス）

商学部 （ 日吉 ）

職名

准教授
Scopus 論文情報  
総論文数: 27 総引用数: 1191 h-index: 15

Click to view the Scopus page. The data was downloaded from Scopus API inMarch 29, 2026, via http://api.elsevier.com and http://www.scopus.com .

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  • Establishment of a zebrafish inbred strain, M-AB, capable of regular breeding and genetic manipulation

    Sadamitsu K., Velilla F., Shinya M., Kashima M., Imai Y., Kawasaki T., Watai K., Hosaka M., Hirata H., Sakai N.

    Scientific Reports 14 （ 1 ）  2024年12月

    　概要を見る

    Inbred strains of organisms are genetically highly uniform and thus useful for life science research. We have previously reported the ongoing generation of the zebrafish IM strain from the India (IND) strain through full sib-pair mating for 16 generations. However, the IM fish laid a small number of offspring and had a short lifespan, implying the need for discreet care in breeding. Here, we report the subsequent establishment of IM strain as well as the generation of a new inbred zebrafish strain, Mishima-AB (M-AB). M-AB was derived from the *AB strain by full sib-pair mating for over 20 generations, which fulfills the general criterion for the establishment of an inbred strain. In contrast to the IM case, maintenance of the M-AB strain by sib-pair mating required almost no special handling. Genome sequencing of IM individuals from the 47th generation and M-AB individuals from the 27th generation revealed that SNP-based genomic heterogeneity across whole-genome nucleotides was 0.008% and 0.011%, respectively. These percentages were much lower than those of the parental IND (0.197%) and *AB (0.086%) strains. These results indicate that the genomes of these inbred strains were highly homogenous. We also demonstrated the successful microinjection of antisense morpholinos, CRISPR/Cas9, and foreign genes into M-AB embryos at the 1-cell stage. Overall, we report the establishment of a zebrafish inbred strain, M-AB, which is capable of regular breeding and genetic manipulation. This strain will be useful for the analysis of genetically susceptible phenotypes such as behaviors, microbiome features and drug susceptibility.

  • High-speed system to generate congenic strains in medaka

    Shinya M., Kimura T., Naruse K.

    Genes and Genetic Systems 98 （ 5 ） 267 - 275 2023年

    ISSN  13417568

    　概要を見る

    The congenic strain, an inbred strain containing a small genomic region from another strain, is a powerful tool to assess the phenotypic effect of polymorphisms and/or mutations in the substituted genomic region. Recent substantial progress in the genetic studies of complex traits increases the necessity of congenic strains and, therefore, a quick breeding system for congenic strains has become increasingly important in model organisms such as mouse and medaka. Traditionally, more than ten generations are necessary to produce a congenic strain. In contrast, a quick method has been reported previously for the mouse, in which the use of genetic markers reduces the required number of backcross generations to about a half that of the traditional method, so that it would take around six generations to obtain a congenic strain. Here, we present an even quicker congenic production system, which takes only about four generations. The system can produce medaka congenic strains having part of the HNI-II (an inbred medaka strain derived from the northern Japanese population, Oryzias sakaizumii) genome in the HdrR-II1 (another inbred strain from the southern Japanese population, O. latipes) background. In this system, the availability of frozen sperm and genotype data of the BC<inf>1</inf> male population makes it possible to start marker-assisted congenic production after obtaining the BC<inf>2</inf> population. Our evaluation revealed that the system could work well to increase the percentage of recipient genome as expected, so that a congenic strain may be obtained in about one year.

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競争的研究費の研究課題 【 表示 ／ 非表示 】

  • メダカ頭蓋顔面形態の個体差形成メカニズムの解明～多型・遺伝子の同定～

    2022年04月
    -
    2026年03月

    文部科学省・日本学術振興会, 科学研究費助成事業, 新屋 みのり, 基盤研究(C), 補助金,  研究代表者

  • メダカ頭蓋顔面形態の多様性～個体差を担う遺伝子を探す～

    2014年04月
    -
    2017年03月

    文部科学省・日本学術振興会, 科学研究費助成事業, 新屋　みのり, 基盤研究(C), 補助金,  研究代表者

    　研究概要を見る

    頭蓋顔面形態は多様性に富む興味深い形質であり、その形成には環境と遺伝要因の両方が関わるとされている。我々は頭蓋顔面形態の多様性形成に関わる遺伝子の同定を目指し、メダカを用いた統計遺伝学的解析を実施した。その結果、形質とゲノム領域との関連を複数見出し、形質L33の関連ゲノム領域を6番染色体の約5Mbpに絞り込むことに成功した。また、形質D29に対する二つの関連ゲノム領域の検証を進めたが、いずれも陰性であった。もう一つの形質V13では、形質自体が環境の影響を受けやすく不安定であることが判明し、解析不能との判断を下した。今後まもなくL33の絞り込みが完了し、遺伝子の同定へと進める予定である。

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担当授業科目 【 表示 ／ 非表示 】

  • 総合教育セミナーＤｂ（Ⅰ類）

    2024年度

  • 総合教育セミナーＤａ（Ⅰ類）

    2024年度

  • 生物学Ⅱ（実験を含む）

    2024年度

  • 生物学Ⅰ（実験を含む）

    2024年度

  • 総合教育セミナーＳ（Ⅰ類）

    2023年度

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