Kawaai, Katsuhiro

写真a

Affiliation

School of Medicine, Center for the Promotion of Interdiciplinary Research in Medicine and Life Scieince(Research Centers, Laboratory of Chiral Biology) ( Shinanomachi )

Position

Senior Assistant Professor (Non-tenured)/Assistant Professor (Non-tenured)

External Links
Scopus Paper Info  
Paper Count: 23 Citation Count: 470 h-index: 12

Click to view the Scopus page. The data was downloaded from Scopus API in September 13, 2026, via http://api.elsevier.com and http://www.scopus.com .

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Career 【 Display / hide 】

  • 2009.04
    -
    2011.03

    科学技術振興機構, カルシウム振動プロジェクト, 研究員

  • 2011.04
    -
    2018.06

    RIKEN, 研究員

  • 2018.07
    -
    2024.09

    Keio University, School of Medicine Collaborative Research Resources (Laboratory of Cell and Tissue Biology), 助教

  • 2024.10
    -
    2026.06

    Keio University, School of Medicine Collaborative Research Resources (Laboratory of Cell and Tissue Biology), 専任講師(学部内)

  • 2026.07
    -
    Present

    Keio University, School of Medicine PIRMS (Laboratory of Chiral Biology), 専任講師(学部内)

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Research Areas 【 Display / hide 】

  • Life Science / Molecular biology

  • Life Science / Neuroscience-general

  • Life Science / Biomaterials

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Research Keywords 【 Display / hide 】

  • 包括脳ネットワーク

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Books 【 Display / hide 】

  • 分子細胞生物学辞典, 第２版

    河合克宏, 東京化学同人, 2008.10

    Scope: 「4.1」「ドーパミントランスポーター」「Parkin」「Pax」「VEGF受容体」「スクランブラーマウス」「ヨタリマウス」「disabled homolog 1」「リーラーマウス」「小脳プルキンエ細胞欠失ミュータントマウス」「スタゲラーマウス」「ナーバスマウス」「pcdマウス」「シナプス後肥厚部」「バーグマングリア」

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Papers 【 Display / hide 】

  • Matrix Metalloproteinase‐13 Is an Unfavorable Prognostic Factor in Chordoma by Digesting Growth Inhibitory Collagens

    Yumiko Oishi, Katsuhiro Kawaai, Ryota Tamura, Yukiko Kuroda, Shinobu Noji, Masahiro Toda, Koichi Matsuo

    Cancer Medicine 15 ( 2 ) e71662 2026.02

    Corresponding author, Accepted

  • Use of a new micropattern tape method to detect chirality shifts in differentiating C2C12 cells

    Qingkai Weng, Takashi Osaka, Hiroaki Onoe, Koki Yoshida, Yukiko Kuroda, Koichi Matsuo, Katsuhiro Kawaai

    PLOS One 20 ( 12 ) e0338032 2025.12

    Last author, Corresponding author, Accepted,  ISSN  1932-6203

    　View Summary

    <jats:p>
    Chirality is an intrinsic property of cells manifested as left-right (LR) asymmetry in terms of cellular morphology and organization, which influences cell behavior, migration, and tissue development. Traditional
    <jats:italic>in vitro</jats:italic>
    methods used to study cell chirality often require complex fabrication methods, limiting their accessibility and reproducibility. Here, we present a novel micropattern tape method that facilitates fabrication of high-quality rectangular micropatterns useful for efficient, high-throughput analysis of cell chirality. Using this method, we characterized chirality of C2C12 myoblasts and MC3T3-E1 osteoblasts, which respectively exhibit clockwise (CW) and counterclockwise (CCW) chirality relative to the long axis of the rectangle. We used the method to analyze how cellular differentiation impacts chirality and observed striking reversal of C2C12 cell chirality upon bone morphogenic protein-2 (BMP2)-induced osteoblastic differentiation. These results demonstrate that our micropattern tape method can effectively detect dynamic change of cell chirality during differentiation.
    </jats:p>

  • Osteoclast visualization: Tartrate-resistant acid phosphatase activity staining using NewFuchsin compatible with non-aqueous mounting and tissue clearing

    Takashi Nakamura, Katsuhiro Kawaai, Yukiko Kuroda, Koichi Matsuo

    MethodsX (Elsevier BV)  14   103136 - 103136 2024.12

    Accepted,  ISSN  2215-0161

  • Developing long bones respond to surrounding tissues by trans-pairing of periosteal osteoclasts and endocortical osteoblasts.

    Yukiko Kuroda, Masaki Yoda, Katsuhiro Kawaai, Motoharu Tatenuma, Toshihide Mizoguchi, Shinichirou Ito, Masataka Kasahara, Yanlin Wu, Hidekazu Takano, Atsushi Momose, Koichi Matsuo

    Development (Cambridge, England) (The Company of Biologists)  151 ( 17 ) dev202194 2024.08

    Accepted,  ISSN  0950-1991

    　View Summary

    Developing long bones alter their shape while maintaining uniform cortical thickness via coordinated activity of bone-forming osteoblasts and bone-resorbing osteoclasts at periosteal and endosteal surfaces, a process we designate trans-pairing. Two types of trans-pairing shift cortical bone in opposite orientations: peri-forming trans-pairing (peri-t-p) increases bone marrow space and endo-forming trans-pairing (endo-t-p) decreases it, via paired activity of bone resorption and formation across the cortex. Here, we focused on endo-t-p in growing bones. Analysis of endo-t-p activity in the cortex of mouse fibulae revealed osteoclasts under the periosteum compressed by muscles and expression of RANKL in periosteal cells of the cambium layer. Furthermore, mature osteoblasts were localized on the endosteum, while preosteoblasts were at the periosteum and within cortical canals. X-ray tomographic microscopy revealed the presence of cortical canals more closely associated with endo- than with peri-t-p. Sciatic nerve transection followed by muscle atrophy and unloading induced circumferential endo-t-p with concomitant spread of cortical canals. Such canals likely supply the endosteum with preosteoblasts from the periosteum under endo-t-p, allowing bone shape to change in response to mechanical stress or nerve injury.

  • Chordoma cells possess bone-dissolving activity at the bone invasion front

    Katsuhiro Kawaai, Yumiko Oishi, Yukiko Kuroda, Ryota Tamura, Masahiro Toda, Koichi Matsuo

    Cellular Oncology (Springer Science and Business Media LLC)  47 ( 5 ) 1663 - 1677 2024.04

    Accepted,  ISSN  2211-3436

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Papers, etc., Registered in KOARA 【 Display / hide 】

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Reviews, Commentaries, etc. 【 Display / hide 】

  • IRBITは脂肪細胞分化初期の細胞増殖を制御している

    関口藍理, 浜田浩一, 佐藤聖子, 高橋透泰, 下田沙弥, 波多野直哉, 河合克宏, 御子柴克彦, 水谷顕洋

    聖マリアンナ医科大学雑誌(Web) (聖マリアンナ医科大学医学会)  44 ( 3 ) 183 (WEB ONLY) - 183 2016.11

    Other, Joint Work,  ISSN  2189-0285

  • IRBITはphosphatidylinositol phosphate kinasesの活性中心と結合する

    安東英明, 廣瀬松美, GAINCHE Laura, 河合克宏, BONNEAU Benjamin, 伊集院壮, 伊藤俊樹, 竹縄忠臣, 御子柴克彦

    日本生化学会大会(Web) 88th 2015

  • Genes associated with long-lasting synaptic enhancement in hippocampal slices after repetitive glutamate stimulation

    T. Tashiro, K. Kawaai, K. Tominaga-Yoshino, N. Taniguchi, H. Tashiro, A. Ogura

    JOURNAL OF NEUROCHEMISTRY (BLACKWELL PUBLISHING)  102   276 - 276 2007.08

    ISSN  0022-3042

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Presentations 【 Display / hide 】

  • Collective chiral alignment of osteoblasts: sensing 3D surface geometry through curvature and torsion

    河合克宏, 黒田有希子, 吉田光輝, 尾上弘晃, 百生敦, 松尾光一

    第44回日本骨代謝学会学術集会, 

    2026.07
    , 

    Oral presentation (general)

  • Homochiral Bilaterality in Skeletal Morphogenesis: from Osteoblasts to Cranial Architecture

    松尾光一, 宇佐美心手, 熊木勘太, 野路しのぶ, 吉田光輝, 尾上弘晃, 百生敦, 黒田有希子, 河合克宏

    第44回日本骨代謝学会学術集会, 

    2026.07
    , 

    Oral presentation (general)

  • Does Brain Asymmetry Shape Frontal Bone Morphology? Micro-CT-based Analysis of the Intracranial Vault Volume Beneath the Mouse Frontal Bone

    宇佐美心手, 野路しのぶ, 黒田有希子, 河合克宏, 松尾光一

    第44回日本骨代謝学会学術集会, 

    2026.07
    , 

    Oral presentation (general)

  • Trans-pairing regulates adaptive bone growth to surrounding tissues: a morphogenetic mechanism driving bone drift

    黒田有希子, 河合克宏, 松尾光一

    第44回日本骨代謝学会学術集会, 

    2026.07
    , 

    Oral presentation (general)

  • Bipixel method: analysis of symmetry and chirality in mesh structures of cortical canals

    熊木勘太, 河合克宏, 野路しのぶ, 黒田有希子, 松尾光一

    第44回日本骨代謝学会学術集会, 

    2026.07
    , 

    Poster presentation

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Research Projects of Competitive Funds, etc. 【 Display / hide 】

  • Elucidation of the mechanisms for bone destruction in chordoma.

    2022.04
    -
    2025.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Principal investigator

    　View Summary

    我々が行った予備実験の結果、脊索腫細胞そのものが、骨浸潤部位において破骨細胞様のふるまいをし、酸性環境を作り出すことで骨破壊を引き起こしている可能性を見出した。本研究では、骨代謝学において蓄積のある破骨細胞研究の知見を元に、「脊索腫細胞自身が直接骨破壊を引き起こす」という仮説を検証することで、脊索腫における骨破壊の機序を解明し、それを標的とした新たな治療戦略の提言を目指す。

  • Understanding the cellular basis of left-right symmetry in the skeleton

    2021.04
    -
    2025.03

    Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), 松尾 光一, 河合 克宏, 黒田 有希子, Grant-in-Aid for Scientific Research (B), No Setting

    　View Summary

    動物の骨格の左右対称性は、歩行や走行、遊泳、飛行の基盤となる。「骨格パターン形成の段階」では、骨芽細胞の細胞キラリティ（互いに鏡像の左手型や右手型細胞のこと）と左右対称性形態形成の関係を解明する。次に「骨の変容成長の段階」では、皮質骨が周囲の臓器の変化に応じて吸収され、対面の皮質骨が形成される「内形成性トランスペアリング」が起きるメカニズムを解明し、鏡像的な骨周囲環境で骨格が左右対称になることを示す。

  • 骨化軟骨を模倣したバイオミメティクスコート材の開発および骨芽細胞動態解析による新規評価法の確立

    2020.10
    -
    2021.09

    (株)JSR, 学術開発プロジェ クト (継続課題), Principal investigator

  • 骨化軟骨を模倣したバイオミメティクスコート材の開発および骨芽細胞動態解析による新規評価法の確立

    2019.10
    -
    2020.09

    (株)JSR, 学術開発プロジェ クト (継続課題), Principal investigator

  • Applicational study of bone formation mechanism based on calcified cartilage

    2019.04
    -
    2022.03

    Keio University, Grants-in-Aid for Scientific Research, KAWAAI Katsuhiro, Grant-in-Aid for Scientific Research (C), No Setting

    　View Summary

    Based on the histological analysis of endochondral ossification, we found that calcified cartilage function as a superior scaffold for bone formation by osteoblasts. So, we performed screening of molecules enhance bone formation from components of calcified cartilage and applied to coating. We identified several candidate molecules and these coating on culture dish significantly enhanced in vitro bone formation by osteoblasts. In addition, we designed a surface micropattern inspired by characteristic structures of calcified cartilage. We also developed assay methods to evaluate the surface properties by using kinetics of osteoblast migration. The micropattern changed in vitro bone formation rate and cell migration speed of osteoblasts. From these examinations, we suggest that the combination of novel coating and surface micropatterns provide effective surface properties for implants to achieve high biological compatibility and secure engraftment.

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Courses Taught 【 Display / hide 】

  • MEDICAL PROFESSIONALISM 3

    2025

  • MEDICAL PROFESSIONALISM 3

    2024

  • MEDICAL PROFESSIONALISM 3

    2023

  • MEDICAL PROFESSIONALISM 3

    2022

  • MEDICAL PROFESSIONALISM 3

    2021

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Courses Previously Taught 【 Display / hide 】

  • Medical Professionalism III (Research Ethics)

    School of Medicine, Keio University

    2018
    -
    Present

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