Citterio, Daniel

写真a

Affiliation

Faculty of Science and Technology, Department of Applied Chemistry ( Yagami )

Position

Professor

Related Websites

External Links
Scopus Paper Info  
Paper Count: 214 Citation Count: 8579 h-index: 47

Click to view the Scopus page. The data was downloaded from Scopus API in April 05, 2026, via http://api.elsevier.com and http://www.scopus.com .

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Career 【 Display / hide 】

  • 2002.11
    -
    2003.09

    Swiss Federal Institute of Technology Zurich (ETHZ), Switzerland, Centre for Chemical Sensors, Senior Research Scientist

  • 2005.02
    -
    2006.01

    Ciba Specialty Chemicals Inc., Basel (Switzerland), Scientific Information Department, Patent Attorney

  • 2006.03
    -
    2007.03

    Keio University Faculty of Science and Technology, Graduate School of Science and Technology, Special Research Associate Professor

  • 2007.04
    -
    2009.03

    Keio University Faculty of Science and Technology, Department of Applied Chemistry, Associate Professor (non-tenured)

  • 2009.04
    -
    2014.03

    Keio University Faculty of Science and Technology, Department of Applied Chemistry, Associate Professor

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Research Areas 【 Display / hide 】

  • Nanotechnology/Materials / Analytical chemistry

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Research Keywords 【 Display / hide 】

  • biosensors

  • chemical sensors

  • functional dyes

  • functional materials

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Books 【 Display / hide 】

  • Applications of Microfluidic Systems in Biology and Medicine

    Yanawut Manmana, Kentaro Yamada, Daniel Citterio, Springer Nature Singapore Pte Ltd., 2024

    Scope: Paper-Based Microfluidics for Point-of-Care Medical Diagnostics / 443-493

  • Applications of Microfluidic Systems in Biology and Medicine

    Yamada Kentaro, Citterio Daniel, Springer, Singapore, 2019.04

    Scope: Paper-Based Microfluidics for Point-of-Care Medical Diagnostics / 353-382

  • Materials for Chemical Sensing

    Citterio Daniel, Springer, 2017

    Scope: (Bio)Chemical Sensors Based on Paper / 29-74

  • Design of Polymeric Platforms for Selective Biorecognition

    Citterio Daniel, Springer, 2015

    Scope: Inkjet Printing of Biomolecules for Biorecognition / 197-235

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Papers 【 Display / hide 】

  • Progress toward real-world diagnostic applications of microfluidic paper-based analytical devices (μPADs)

    Tanifuji Y., Citterio D.

    Lab on A Chip 26 ( 5 ) 1191 - 1218 2026.03

    ISSN  14730197

    　View Summary

    Since their first report in 2007, microfluidic paper-based analytical devices (μPADs) have continued to gain attention as promising tools for point-of-care diagnostics due to their low cost, portability, ease of operation, and design flexibility. This review summarizes and discusses recent advances in the field, mostly based on works published between 2017 and 2025, with a focus on progress and remaining challenges in bridging the gap between proof-of-concept demonstrations in academic laboratories and real-world implementation. Special emphasis is placed on devices validated with clinical samples and capable of true sample-in–answer-out operation. To comprehensively assess recent developments, nearly one hundred reported examples were analysed not only in terms of analytical figures of merit but also with respect to practical criteria such as real-sample testing, long-term storage stability, the need for off-device sample pretreatment, reagent handling complexity, time-control requirements, and the number of operation steps. In parallel, topics of ongoing academic interest are highlighted, including automated sequential reagent delivery, strategies for accelerating liquid flow, and robust signal readout methods going beyond purely qualitative approaches to enhance assay sensitivity, precision, rapidity, and instrument-free usability. Finally, the review introduces emerging analytical technologies newly integrated into μPAD platforms, such as surface-enhanced Raman scattering (SERS), bioluminescence, CRISPR-based assays, and machine learning-driven data interpretation, which further expand the analytical capabilities and scope of μPADs.

  • Integrating smartphone optical sensors with paper-based analytical devices: a future standalone platform for onsite detection and analysis

    Manmana Y., Chetry B., Dutta S., Nath P., Citterio D.

    Trac Trends in Analytical Chemistry 196 2026.03

    ISSN  01659936

    　View Summary

    The beginning of this century has witnessed the emergence of many disruptive technologies changing the ways of sensing target analytes. Among these, smartphone-enabled analytical devices (SEADs) have gained attention as a promising technology, particularly in resource-limited settings. SEADs are recognized for being portable, cost-effective, user-friendly, and reliable platforms for detecting a wide range of analytes. In parallel, paper-based analytical devices (PADs) primarily relying on colorimetric or fluorescence signal transduction, have also attracted significant research interest across various applications. This review highlights the potential of integrating these two emerging analytical platforms, a combination that could revolutionize future onsite sensing. An overview of SEADs and PADs is provided, with focus on recent developments in optical detection. Advantages and limitations of integrating smartphones with PADs in different scenarios are presented. Additionally, recent applications of smartphone-coupled PADs are presented. Finally, key challenges associated with merging these two technologies are discussed.

  • Portable Potentiometric Aptasensor Using an Ion Flux Polymer Membrane for Rapid Detection of Salmonella Typhimurium in Meat and Water

    Preechakasedkit P., Pulsrikarn C., Nuanualsuwan S., Auanphui C., Henry C.S., Citterio D., Ruecha N.

    Food Analytical Methods 19 ( 2 )  2026.02

    ISSN  19369751

    　View Summary

    The rapid and accurate detection of foodborne pathogens, particularly Salmonella Typhimurium (S. Typhimurium), is essential for ensuring food quality and safety. This work presents a portable, label-free potentiometric aptasensor using an ion flux polymer membrane for the sensitive and rapid detection of S. Typhimurium in meat and water, operated via a smartphone-based potentiostat. The sensor employs a specific aptamer immobilized on a solid-state contact ion-selective electrode, featuring a cationic marker and a carboxylated poly(vinyl chloride) membrane modified on a laser-induced graphene electrode. Binding of outer membrane protein D from S. Typhimurium to its aptamer hinders cation flux across the membrane, generating measurable potential changes that enable quantitative detection within 8 min. The assay offered a linear range of 1–10,000 CFU/mL, a detection limit of 1 CFU/mL, and a sensitivity of 16.572 mV/decade. Sensor performance was validated in various meat and water matrices, including groundwater, tap water, drinking water, pork, and chicken, yielding results consistent with ISO 19250:2010 and a commercial lateral flow immunoassay. Recovery rates from spiked meat samples ranged from 96 to 108%, with relative standard deviations below 5.7%, demonstrating good accuracy in complex matrices. The aptasensor also exhibited excellent specificity toward S. Typhimurium and retained over 90% of its sensitivity after three weeks of storage, confirming its good storage stability and practical applicability. These findings indicate that the proposed portable aptasensor is well suited for routine on-site detection of S. Typhimurium in food products and represents a valuable tool for rapid food safety monitoring.

  • Two approaches for mercury determination in environmental and food monitoring via catalytic fluorometry of Au-Hg amalgamation using a laser-printed paper sensor and microcentrifuge tube test kit

    Malahom N., Boonthod S., Veerasup N., Pajarean C., Prakobkij A., Anutrasakda W., Citterio D., Amatatongchai M., Nacapricha D., Jarujamrus P.

    Microchimica Acta 192 ( 9 )  2025.09

    ISSN  00263672

    　View Summary

    Two selective methods were developed for detecting mercury ions (Hg<sup>2</sup>⁺) in food and water samples using catalytic fluorometry based on Au-Hg amalgamation with enhanced peroxidase-like activity. These approaches include (i) a laser-printed microfluidic paper-based analytical device (LP-µPAD) and (ii) a paper-based microcentrifuge tube test kit. The LP-µPAD was fabricated via commercial laser printing and integrated gold nanoparticles (AuNPs) with o-phenylenediamine (OPD) and hydrogen peroxide (H₂O₂). The test kit enabled Hg<sup>2</sup>⁺ detection in food by reducing Hg<sup>2</sup>⁺ to Hg⁰ with stannous chloride, facilitating Au-Hg amalgam formation in the detection zone. Both approaches utilized the Au-Hg amalgam to catalyze H₂O₂-mediated oxidation of OPD, generating fluorescent 2,3-diaminophenazine (DAP), further enhancing fluorescence intensity in proportion to Hg<sup>2</sup>⁺ concentrations. The LP-µPAD exhibited a detection range of 3.0–20.0 µg L⁻<sup>1</sup> with a limit of detection (LOD) of 1.65 µg L⁻<sup>1</sup>, whereas the test kit provided a detection range of 0.1–1.0 mg L⁻<sup>1</sup> with an LOD of 0.08 mg L⁻<sup>1</sup>. Both sensors showed high selectivity for Hg<sup>2</sup>⁺ over other ions and performed well in real sample analyses, aligning closely with results from conventional methods.

  • A Paper-Based Colorimetric Sensor for Tumor Marker CA-125 Using Aptamer-Enhanced Ni–MnFe Layered Double Hydroxide Nanozymes

    Prakobkij A., Kitchawengkul N., Anutrasakda W., Vilaivan T., Wanram S., Amatatongchai M., Citterio D., Jarujamrus P.

    ACS Applied Nano Materials 8 ( 29 ) 14556 - 14566 2025.07

    　View Summary

    Cancer antigen 125 (CA-125) is a glycoprotein associated with cholangiocarcinoma, making it a valuable biomarker for diagnosis. However, conventional detection methods have limitations due to their reliance on antibodies for specificity and natural enzymes as labels for signal amplification. Both are costly and unstable under extreme pH conditions, and enzymes exhibit a low catalytic efficiency. Addressing these limitations could enhance the diagnostic accuracy of CA-125 detection in cholangiocarcinoma diagnosis. In this work, Ni-MnFe-layered double hydroxides (Ni-MnFe-LDHs) conjugated with an aptamer (Ni-MnFe-LDHs@aptamer) are demonstrated as a superior peroxidase mimic to enhance the sensitivity for the colorimetric detection of CA-125 on a paper-based analytical device (PAD) platform. Ni-MnFe-LDHs exhibited efficient peroxidase-like activity, catalyzing the oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) into an intense blue product in the presence of hydrogen peroxide (H<inf>2</inf>O<inf>2</inf>). Upon modification with a CA 125-specific aptamer, Ni-MnFe-LDHs became more dispersed due to electrostatic repulsion, exposing more active sites and generating more hydroxyl radicals (<sup>·</sup>OH). Moreover, π–π stacking and hydrogen bond interactions between the aptamer and TMB increased the substrate affinity of Ni-MnFe-LDHs, thereby enhancing their catalytic performance. This resulted in a darker blue signal in the absence of CA-125. When CA-125 was present in the sample, it was captured by the aptamer on Ni-MnFe-LDHs, resulting in a decreased blue color signal. Using CA-125 as the target analyte, a linear relationship between the scanner-recorded signal intensity and analyte concentration was observed in the range of 10–25 U/mL. The results obtained from real human sample application using the developed method were consistent with those from a clinical laboratory. This method can be easily implemented and holds great potential as a prototype for various diagnostic applications, especially for detecting biomarkers in serum or plasma samples. It offers significant benefits for point-of-care testing.

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Papers, etc., Registered in KOARA 【 Display / hide 】

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Reviews, Commentaries, etc. 【 Display / hide 】

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Presentations 【 Display / hide 】

  • Barcode Approaches for Semiquantitative Signal Readout of Colorimetric Microfluidic Paper-Based Analytical Devices

    Daniel Citterio

    [International presentation]  Pittcon 2026, 

    2026.03
    , 

    Oral presentation (invited, special), The Pittsburgh Conference

  • Paper-based analytical devices spanning the range from utmost simplicity to highest sensitivity

    Daniel Citterio

    [International presentation]  Pacifichem 2025 (Honolulu) , 

    2025.12
    , 

    Oral presentation (invited, special), American Chemical Society

  • CRISPR/Cas-Assisted Assays Integrated into Low-Cost Analytical Devices for Point-of-Need Applications

    Daniel Citterio

    [International presentation]  Pacifichem 2025 (Honolulu) , 

    2025.12
    , 

    Oral presentation (invited, special), American Chemical Society

  • Barcode Strategies for Semiquantitative Signal Readout of Colorimetric Microfluidic Paper-Based Analytical Devices

    Daniel Citterio

    [International presentation]  Hub of Knowledge in Chemical Sensor and Biosensor Technology International Conference 2025 (Bangkok) , 

    2025.11
    , 

    Oral presentation (invited, special)

  • 1D and 2D Barcode Strategies for Semiquantitative Signal Readout of Colorimetric Microfluidic Paper-Based Analytical Devices

    Daniel Citterio

    [International presentation]  Lab-on-a-Chip & Microfluidics Asia 2025 (Narita) , 

    2025.10
    , 

    Oral presentation (invited, special), SelectBIO

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Research Projects of Competitive Funds, etc. 【 Display / hide 】

  • 核酸抽出から検出までを統合した高感度簡易核酸分析3D印刷ピペットチップ型デバイス

    2025.04
    -
    2028.03

    基盤研究(B), Principal investigator

  • CRISPR/Casを用いた事前増幅不要で高感度核酸検出可能な紙基板分析デバイス

    2022.04
    -
    2025.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, 基盤研究(B), Principal investigator

  • Microfluidic thread-based sensor for the detection of therapeutic antibodies in blood at point-of-care

    2018.04
    -
    2021.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Principal investigator

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Awards 【 Display / hide 】

  • Award for Creative Work

    Daniel Citterio, 2022.03, The Chemical Society of Japan, Development of Functional Dyes and Paper-Based Analytical Devices for Chemical and Biochemical Sensing

    Type of Award： Award from Japanese society, conference, symposium, etc.

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Courses Taught 【 Display / hide 】

  • BACHELOR'S THESIS

    2026

  • PRACTICAL ENGLISH FOR APPLIED CHEMISTRY

    2026

  • FUNDAMENTAL ANALYTICAL CHEMISTRY

    2026

  • ADVANCED COURSE OF EMERGING SCIENCE A

    2026

  • SEMINAR IN EMERGING PHYSICO-CHEMISTRY 2

    2026

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Courses Previously Taught 【 Display / hide 】

  • Practical English for Applied Chemistry

    Keio University

    2016.04
    -
    2017.03
    , 

    Full academic year, Lecture, Within own ｆaculty

  • Laboratories in Basic Chemistry

    Keio University

    2016.04
    -
    2017.03
    , 

    Autumn Semester, Laboratory work/practical work/exercise, Within own ｆaculty

  • Laboratories in Applied Chemistry

    Keio University

    2016.04
    -
    2017.03
    , 

    Spring Semester, Laboratory work/practical work/exercise, Within own ｆaculty

  • Introduction to Functional Materials

    Keio University

    2016.04
    -
    2017.03
    , 

    Spring Semester, Lecture, Within own ｆaculty

  • Practical Instrumental Analysis

    Keio University

    2016.04
    -
    2017.03
    , 

    Spring Semester, Lecture, Within own ｆaculty

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Memberships in Academic Societies 【 Display / hide 】

  • Chemical Society of Japan (CSJ), 

    2006.04
    -
    Present

  • Japan Society for Analytical Chemistry (JSAC), 

    2006.04
    -
    Present

  • American Chemical Society (ACS), 

    2007.03
    -
    Present

  • Royal Society of Chemistry (RSC) (Fellow), 

    2016.02
    -
    Present
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Committee Experiences 【 Display / hide 】

  • 2017.04
    -
    Present

    Permanent Steering Committee of Europt(r)ode, Europt(r)ode

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