慶應義塾研究者情報データベース

経歴 【 表示 ／ 非表示 】

経歴 【 表示 ／ 非表示 】

• 2002年11月

  -

  2003年09月

  スイス連邦工科大学, 化学センサーセンター, 上級研究員

• 2005年02月

  -

  2006年01月

  チバ・スペシャルティ・ケミカルズ株式会社（スイス）, 特許情報管理部, 特許弁理士

• 2006年03月

  -

  2007年03月

  慶應義塾大学理工学部, 大学院理工学研究科, 特別研究助教授

• 2007年04月

  -

  2009年03月

  慶應義塾大学理工学部, 応用化学科, 准教授（有期）

• 2009年04月

  -

  2014年03月

  慶應義塾大学理工学部, 応用化学科, 准教授

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研究分野 【 表示 ／ 非表示 】

研究分野 【 表示 ／ 非表示 】

• ナノテク・材料 / 分析化学

研究キーワード 【 表示 ／ 非表示 】

研究キーワード 【 表示 ／ 非表示 】

• バイオセンサ

• 化学センサ

• 機能性色素

• 機能材料

著書 【 表示 ／ 非表示 】

著書 【 表示 ／ 非表示 】

• Applications of Microfluidic Systems in Biology and Medicine

  Yanawut Manmana, Kentaro Yamada, Daniel Citterio, Springer Nature Singapore Pte Ltd., 2024年

  担当範囲: Paper-Based Microfluidics for Point-of-Care Medical Diagnostics / 443-493

• Applications of Microfluidic Systems in Biology and Medicine

  山田　健太郎、チッテリオ　ダニエル, Springer, Singapore, 2019年04月

  担当範囲: Paper-Based Microfluidics for Point-of-Care Medical Diagnostics / 353-382

• Materials for Chemical Sensing

  チッテリオ　 ダニエル, Springer, 2017年

  担当範囲: (Bio)Chemical Sensors Based on Paper / 29-74

• Design of Polymeric Platforms for Selective Biorecognition

  チッテリオ　 ダニエル, Springer, 2015年

  担当範囲: Inkjet Printing of Biomolecules for Biorecognition / 197-235

論文 【 表示 ／ 非表示 】

論文 【 表示 ／ 非表示 】

• Progress toward real-world diagnostic applications of microfluidic paper-based analytical devices (μPADs)

  Tanifuji Y., Citterio D.

  Lab on A Chip 26 （ 5 ） 1191 - 1218 2026年03月

  ISSN  14730197

  　概要を見る

  Since their first report in 2007, microfluidic paper-based analytical devices (μPADs) have continued to gain attention as promising tools for point-of-care diagnostics due to their low cost, portability, ease of operation, and design flexibility. This review summarizes and discusses recent advances in the field, mostly based on works published between 2017 and 2025, with a focus on progress and remaining challenges in bridging the gap between proof-of-concept demonstrations in academic laboratories and real-world implementation. Special emphasis is placed on devices validated with clinical samples and capable of true sample-in–answer-out operation. To comprehensively assess recent developments, nearly one hundred reported examples were analysed not only in terms of analytical figures of merit but also with respect to practical criteria such as real-sample testing, long-term storage stability, the need for off-device sample pretreatment, reagent handling complexity, time-control requirements, and the number of operation steps. In parallel, topics of ongoing academic interest are highlighted, including automated sequential reagent delivery, strategies for accelerating liquid flow, and robust signal readout methods going beyond purely qualitative approaches to enhance assay sensitivity, precision, rapidity, and instrument-free usability. Finally, the review introduces emerging analytical technologies newly integrated into μPAD platforms, such as surface-enhanced Raman scattering (SERS), bioluminescence, CRISPR-based assays, and machine learning-driven data interpretation, which further expand the analytical capabilities and scope of μPADs.

  • Access to Document (DOI)

• Integrating smartphone optical sensors with paper-based analytical devices: a future standalone platform for onsite detection and analysis

  Manmana Y., Chetry B., Dutta S., Nath P., Citterio D.

  Trac Trends in Analytical Chemistry 196 2026年03月

  ISSN  01659936

  　概要を見る

  The beginning of this century has witnessed the emergence of many disruptive technologies changing the ways of sensing target analytes. Among these, smartphone-enabled analytical devices (SEADs) have gained attention as a promising technology, particularly in resource-limited settings. SEADs are recognized for being portable, cost-effective, user-friendly, and reliable platforms for detecting a wide range of analytes. In parallel, paper-based analytical devices (PADs) primarily relying on colorimetric or fluorescence signal transduction, have also attracted significant research interest across various applications. This review highlights the potential of integrating these two emerging analytical platforms, a combination that could revolutionize future onsite sensing. An overview of SEADs and PADs is provided, with focus on recent developments in optical detection. Advantages and limitations of integrating smartphones with PADs in different scenarios are presented. Additionally, recent applications of smartphone-coupled PADs are presented. Finally, key challenges associated with merging these two technologies are discussed.

  • Access to Document (DOI)

• Portable Potentiometric Aptasensor Using an Ion Flux Polymer Membrane for Rapid Detection of Salmonella Typhimurium in Meat and Water

  Preechakasedkit P., Pulsrikarn C., Nuanualsuwan S., Auanphui C., Henry C.S., Citterio D., Ruecha N.

  Food Analytical Methods 19 （ 2 ）  2026年02月

  ISSN  19369751

  　概要を見る

  The rapid and accurate detection of foodborne pathogens, particularly Salmonella Typhimurium (S. Typhimurium), is essential for ensuring food quality and safety. This work presents a portable, label-free potentiometric aptasensor using an ion flux polymer membrane for the sensitive and rapid detection of S. Typhimurium in meat and water, operated via a smartphone-based potentiostat. The sensor employs a specific aptamer immobilized on a solid-state contact ion-selective electrode, featuring a cationic marker and a carboxylated poly(vinyl chloride) membrane modified on a laser-induced graphene electrode. Binding of outer membrane protein D from S. Typhimurium to its aptamer hinders cation flux across the membrane, generating measurable potential changes that enable quantitative detection within 8 min. The assay offered a linear range of 1–10,000 CFU/mL, a detection limit of 1 CFU/mL, and a sensitivity of 16.572 mV/decade. Sensor performance was validated in various meat and water matrices, including groundwater, tap water, drinking water, pork, and chicken, yielding results consistent with ISO 19250:2010 and a commercial lateral flow immunoassay. Recovery rates from spiked meat samples ranged from 96 to 108%, with relative standard deviations below 5.7%, demonstrating good accuracy in complex matrices. The aptasensor also exhibited excellent specificity toward S. Typhimurium and retained over 90% of its sensitivity after three weeks of storage, confirming its good storage stability and practical applicability. These findings indicate that the proposed portable aptasensor is well suited for routine on-site detection of S. Typhimurium in food products and represents a valuable tool for rapid food safety monitoring.

  • Access to Document (DOI)

• Two approaches for mercury determination in environmental and food monitoring via catalytic fluorometry of Au-Hg amalgamation using a laser-printed paper sensor and microcentrifuge tube test kit

  Malahom N., Boonthod S., Veerasup N., Pajarean C., Prakobkij A., Anutrasakda W., Citterio D., Amatatongchai M., Nacapricha D., Jarujamrus P.

  Microchimica Acta 192 （ 9 ）  2025年09月

  ISSN  00263672

  　概要を見る

  Two selective methods were developed for detecting mercury ions (Hg²⁺) in food and water samples using catalytic fluorometry based on Au-Hg amalgamation with enhanced peroxidase-like activity. These approaches include (i) a laser-printed microfluidic paper-based analytical device (LP-µPAD) and (ii) a paper-based microcentrifuge tube test kit. The LP-µPAD was fabricated via commercial laser printing and integrated gold nanoparticles (AuNPs) with o-phenylenediamine (OPD) and hydrogen peroxide (H₂O₂). The test kit enabled Hg²⁺ detection in food by reducing Hg²⁺ to Hg⁰ with stannous chloride, facilitating Au-Hg amalgam formation in the detection zone. Both approaches utilized the Au-Hg amalgam to catalyze H₂O₂-mediated oxidation of OPD, generating fluorescent 2,3-diaminophenazine (DAP), further enhancing fluorescence intensity in proportion to Hg²⁺ concentrations. The LP-µPAD exhibited a detection range of 3.0–20.0 µg L⁻¹ with a limit of detection (LOD) of 1.65 µg L⁻¹, whereas the test kit provided a detection range of 0.1–1.0 mg L⁻¹ with an LOD of 0.08 mg L⁻¹. Both sensors showed high selectivity for Hg²⁺ over other ions and performed well in real sample analyses, aligning closely with results from conventional methods.

  • Access to Document (DOI)

• A Paper-Based Colorimetric Sensor for Tumor Marker CA-125 Using Aptamer-Enhanced Ni–MnFe Layered Double Hydroxide Nanozymes

  Prakobkij A., Kitchawengkul N., Anutrasakda W., Vilaivan T., Wanram S., Amatatongchai M., Citterio D., Jarujamrus P.

  ACS Applied Nano Materials 8 （ 29 ） 14556 - 14566 2025年07月

  　概要を見る

  Cancer antigen 125 (CA-125) is a glycoprotein associated with cholangiocarcinoma, making it a valuable biomarker for diagnosis. However, conventional detection methods have limitations due to their reliance on antibodies for specificity and natural enzymes as labels for signal amplification. Both are costly and unstable under extreme pH conditions, and enzymes exhibit a low catalytic efficiency. Addressing these limitations could enhance the diagnostic accuracy of CA-125 detection in cholangiocarcinoma diagnosis. In this work, Ni-MnFe-layered double hydroxides (Ni-MnFe-LDHs) conjugated with an aptamer (Ni-MnFe-LDHs@aptamer) are demonstrated as a superior peroxidase mimic to enhance the sensitivity for the colorimetric detection of CA-125 on a paper-based analytical device (PAD) platform. Ni-MnFe-LDHs exhibited efficient peroxidase-like activity, catalyzing the oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) into an intense blue product in the presence of hydrogen peroxide (H<inf>2</inf>O<inf>2</inf>). Upon modification with a CA 125-specific aptamer, Ni-MnFe-LDHs became more dispersed due to electrostatic repulsion, exposing more active sites and generating more hydroxyl radicals (^·OH). Moreover, π–π stacking and hydrogen bond interactions between the aptamer and TMB increased the substrate affinity of Ni-MnFe-LDHs, thereby enhancing their catalytic performance. This resulted in a darker blue signal in the absence of CA-125. When CA-125 was present in the sample, it was captured by the aptamer on Ni-MnFe-LDHs, resulting in a decreased blue color signal. Using CA-125 as the target analyte, a linear relationship between the scanner-recorded signal intensity and analyte concentration was observed in the range of 10–25 U/mL. The results obtained from real human sample application using the developed method were consistent with those from a clinical laboratory. This method can be easily implemented and holds great potential as a prototype for various diagnostic applications, especially for detecting biomarkers in serum or plasma samples. It offers significant benefits for point-of-care testing.

  • Access to Document (DOI)

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KOARA（リポジトリ）収録論文等 【 表示 ／ 非表示 】

KOARA（リポジトリ）収録論文等 【 表示 ／ 非表示 】

• CRISPR/Casによるシグナル増幅を利用した折り紙イムノアッセイデバイス

  Citterio, Daniel

  学事振興資金研究成果実績報告書 （慶應義塾大学）  2023年

• 血中抗体医薬品のPOC分析を可能にするマイクロ流体糸基板センサー

  Citterio, Daniel

  科学研究費補助金研究成果報告書 2021年

• Mgイオンはエネルギー代謝を制御する新規な細胞内情報伝達物質か？

  Daniel, Citterio

  科学研究費補助金研究成果報告書 2018年

• 細胞・臓器イメージング用新規化学センシング分子試薬の開発

  Citterio, Daniel

  科学研究費補助金研究成果報告書 2011年

総説・解説等 【 表示 ／ 非表示 】

総説・解説等 【 表示 ／ 非表示 】

• Introduction to ‘Paper-Based Point-of-Care Diagnostics’

  Citterio D., Paixão T.R.L.C., de Araujo W.R.

  Sensors and Diagnostics 4 （ 12 ） 1045 - 1046 2025年12月

  　概要を見る

  Daniel Citterio, Thiago Paixão and William Reis de Araujo introduce the Sensors & Diagnostics themed collection on ‘Paper-Based Point-of-Care Diagnostics’.

  • Access to Document (DOI)

• Outstanding Reviewers for Sensors &amp; Diagnostics in 2022

  Cash K.J., Citterio D., Hisamoto H., Lu G., Tost J., Vidal E.M., Rulka A., Titmus V.

  Sensors and Diagnostics 2 （ 4 ）  2023年07月

  • Access to Document (DOI)

• Editorial: Chemical Sensors for Biomedical Use

  Xie X., Citterio D., Chumbimuni-Torres K., Xue M., Wang X.

  Frontiers in Chemistry （Frontiers in Chemistry）  9 2021年05月

  • Access to Document (DOI)

• Equipment-free Detection of K⁺ on Paper

  Soda Y., Citterio D., Bakker E.

  Chimia （Chimia）  73 （ 11 ）  2019年

  ISSN  00094293

  • Access to Document (DOI)

• Paper-based analytical devices

  Citterio D., Kaneta T.

  Analytical Sciences （Analytical Sciences）  34 （ 1 ） 5 - 6 2018年

  ISSN  09106340

  • Access to Document (DOI)

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• Barcode Approaches for Semiquantitative Signal Readout of Colorimetric Microfluidic Paper-Based Analytical Devices

  チッテリオ　ダニエル

  [国際会議]  Pittcon 2026, 

  2026年03月

  , 

  口頭発表（招待・特別）, The Pittsburgh Conference

• Paper-based analytical devices spanning the range from utmost simplicity to highest sensitivity

  チッテリオ　ダニエル

  [国際会議]  Pacifichem 2025 （Honolulu） , 

  2025年12月

  , 

  口頭発表（招待・特別）, American Chemical Society

• CRISPR/Cas-Assisted Assays Integrated into Low-Cost Analytical Devices for Point-of-Need Applications

  チッテリオ　ダニエル

  [国際会議]  Pacifichem 2025 （Honolulu） , 

  2025年12月

  , 

  口頭発表（招待・特別）, American Chemical Society

• Barcode Strategies for Semiquantitative Signal Readout of Colorimetric Microfluidic Paper-Based Analytical Devices

  チッテリオ　ダニエル

  [国際会議]  Hub of Knowledge in Chemical Sensor and Biosensor Technology International Conference 2025 （Bangkok） , 

  2025年11月

  , 

  口頭発表（招待・特別）

• 1D and 2D Barcode Strategies for Semiquantitative Signal Readout of Colorimetric Microfluidic Paper-Based Analytical Devices

  チッテリオ　ダニエル

  [国際会議]  Lab-on-a-Chip & Microfluidics Asia 2025 （Narita） , 

  2025年10月

  , 

  口頭発表（招待・特別）, SelectBIO

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競争的研究費の研究課題 【 表示 ／ 非表示 】

競争的研究費の研究課題 【 表示 ／ 非表示 】

• 核酸抽出から検出までを統合した高感度簡易核酸分析3D印刷ピペットチップ型デバイス

  2025年04月

  -

  2028年03月

  ﾁｯﾃﾘｵ, ﾀﾞﾆｴﾙ, 基盤研究(B), 補助金,  研究代表者

• CRISPR/Casを用いた事前増幅不要で高感度核酸検出可能な紙基板分析デバイス

  2022年04月

  -

  2025年03月

  文部科学省・日本学術振興会, 科学研究費助成事業, チッテリオ ダニエル, 基盤研究(B), 補助金,  研究代表者

• 血中抗体医薬品のPOC分析を可能にするマイクロ流体糸基板センサー

  2018年04月

  -

  2021年03月

  文部科学省・日本学術振興会, 科学研究費助成事業, チッテリオ,　ダニエル, 基盤研究(B), 補助金,  研究代表者

受賞 【 表示 ／ 非表示 】

受賞 【 表示 ／ 非表示 】

• 学術賞

  チッテリオ　ダニエル, 2022年03月, 日本化学会, 化学・生化学センシングのため機能性色素および紙基板分析デバイスの開発

  受賞区分： 国内学会・会議・シンポジウム等の賞

担当授業科目 【 表示 ／ 非表示 】

担当授業科目 【 表示 ／ 非表示 】

• 卒業研究

  2026年度

• 応用化学系英語

  2026年度

• 分析化学基礎

  2026年度

• 創発サイエンス特論Ａ

  2026年度

• 創発理化学輪講２

  2026年度

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担当経験のある授業科目 【 表示 ／ 非表示 】

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• 応用化学系英語

  慶應義塾

  2016年04月

  -

  2017年03月

  , 

  通年, 講義, 専任

• 基礎化学実験

  慶應義塾

  2016年04月

  -

  2017年03月

  , 

  秋学期, 実習・実験, 専任

• 応用化学実験

  慶應義塾

  2016年04月

  -

  2017年03月

  , 

  春学期, 実習・実験, 専任

• 機能物質概論

  慶應義塾

  2016年04月

  -

  2017年03月

  , 

  春学期, 講義, 専任

• 機器分析総論

  慶應義塾

  2016年04月

  -

  2017年03月

  , 

  春学期, 講義, 専任

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所属学協会 【 表示 ／ 非表示 】

所属学協会 【 表示 ／ 非表示 】

• 日本化学会, 

  2006年04月

  -

  継続中

• 日本分析化学会, 

  2006年04月

  -

  継続中

• 米国化学会, 

  2007年03月

  -

  継続中

• 英国王立化学協会 (RSC) (フェロー), 

  2016年02月

  -

  継続中

委員歴 【 表示 ／ 非表示 】

委員歴 【 表示 ／ 非表示 】

• 2017年04月

  -

  継続中

  Permanent Steering Committee of Europt(r)ode, Europt(r)ode