KEIO RESEARCHERS INFORMATION SYSTEM

Career 【 Display / hide 】

Career 【 Display / hide 】

• 1983.10

  -

  1990.09

  長崎大学, 工学部工業化学科, 助手

• 1990.10

  -

  1992.03

  京都大学, 工学部高分子学科, 助手

• 1992.04

  -

  2000.03

  東京工業大学, 生命理工学部生体分子学科, 助教授

• 2000.04

  -

  2002.03

  慶應義塾大学, 理工学部応用化学科, 助教授

• 2000.04

  -

  Present

  慶應義塾大学, 理工学研究科, 委員

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Academic Background 【 Display / hide 】

Academic Background 【 Display / hide 】

• 1981.03

  Kyushu University, Faculty of Engineering, 応用化学科

  University, Graduated

• 1983.03

  Kyushu University, Graduate School, Division of Integrated Science an, 分子工学専攻

  Graduate School, Completed, Master's course

• 1983.09

  Kyushu University, Graduate School, Division of Integrated Science an, 分子工学専攻博士課

  Graduate School, Withdrawal before completion, Doctoral course

Academic Degrees 【 Display / hide 】

Academic Degrees 【 Display / hide 】

• 工学, Kyoto University, Dissertation, 1990.11

Research Areas 【 Display / hide 】

Research Areas 【 Display / hide 】

• Manufacturing Technology (Mechanical Engineering, Electrical and Electronic Engineering, Chemical Engineering) / Electron device and electronic equipment (Functional Materials/Device)

• Nanotechnology/Materials / Nanobioscience (Nano Materials/Nano Bioscience)

• Nanotechnology/Materials / Bio chemistry (Chemistry Related to Living Body)

• Nanotechnology/Materials / Chemical biology

• Life Science / Biomedical engineering (Biomedical Engineering/Biological Material Studies)

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Research Keywords 【 Display / hide 】

Research Keywords 【 Display / hide 】

• ドラッグデリバリーシステム

• ペプチドアプタマー

• 多糖

• 生体膜モデル

• 糖鎖ライブラリー

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Research Themes 【 Display / hide 】

Research Themes 【 Display / hide 】

• Development of infection inhibitor and detection technology for influenza virus, 

  2015

  -

  Present

• 多糖ナノ粒子の作製とドラッグデリバリーシステムへの応用, 

  2000

  -

  Present

• ファージライブラリー法による糖鎖結合性ペプチドおよび糖鎖ミミックペプチドの開発, 

  1999

  -

  Present

• Analyses of cell function based on the novel glycomics using saccharide primer method, 

  1998

  -

  Present

• 生体膜モデルを用いた糖脂質の機能評価, 

  1992

  -

  Present

Proposed Theme of Joint Research 【 Display / hide 】

Proposed Theme of Joint Research 【 Display / hide 】

• ウイルス検出のための認識分子の開発

  Interested in joint research with industry (including private organizations, etc.),  Desired form： Cooperative Research

  　View Summary

  ペプチドアプタマーと糖鎖ライブラリーを用いた病原体の検出手法の開発

• ムコ多糖症診断基質の開発

  Interested in joint research with industry (including private organizations, etc.),  Desired form： Cooperative Research

  　View Summary

  糖鎖ライブラリーを用いたムコ多糖症診断基質の開発

• 多糖ナノ粒子を用いた細胞との相互作用

  Interested in joint research with industry (including private organizations, etc.),  Desired form： Cooperative Research

  　View Summary

  キトサン／ヒアルロン酸／コンドロイチン硫酸等の多糖により形成される多糖ナノ粒子による細胞機能制御

Books 【 Display / hide 】

Books 【 Display / hide 】

• Influenza Virus

  Matsubara T., Sato T., Diamond Electrodes: Fundamentals and Applications, 2022.01

  　View Summary

  The rapid diagnosis of patients in clinical practice is important for anti-influenza therapy. However, since the sensitivity of conventional rapid diagnostic test kits is low, false-negative results are often produced. Therefore, simple and convenient test kits with high sensitivity are needed in clinical practice. In this chapter, we describe the construction of a boron-doped diamond (BDD) electrode that terminates with a receptor (sialic acid-containing oligosaccharide chain)-mimicking peptide as well as its performance in the electrochemical detection of human and avian influenza viruses (IFVs).

  • Access to Document (DOI)

• 生命高分子科学入門

  西村　紳一郎、畑中　研一、佐藤　智典、和田　健彦, 講談社サイエンティフィク, 1999

• 細胞膜に働く人工細胞?人工ウイルスと人工ワクチン

  佐藤 智典, 膜は生きている（日本化学会編）、大日本図書, 1993

• 第４版新実験化学講座 27 生物有機

  佐藤智典、砂本順三, 第４版新実験化学講座 27 生物有機、, 1991

  Scope: 141-148

• Koubunshi to Iryou ( Polymers and Medicine), Mita Press, Tokyo

  T. Sato, J. Sunamoto, 1989

  Scope: 544-577

Papers 【 Display / hide 】

Papers 【 Display / hide 】

• Effects of the Magnetic Orientation of M13 Bacteriophage on Phage Display Selection

  Wang S., Uchida N., Ueno K., Matsubara T., Sato T., Aida T., Ishida Y.

  Chemistry - A European Journal （Chemistry - A European Journal)  29 （ 63 ）  2023.11

  ISSN  09476539

  　View Summary

  Although phage display selection using a library of M13 bacteriophage has become a powerful tool for finding peptides that bind to target materials on demand, a remaining concern of this method is the interference by the M13 main body, which is a huge filament >103 times larger than the displayed peptide, and therefore would nonspecifically adhere to the target or sterically inhibit the binding of the displayed peptide. Meanwhile, filamentous phages are known to be orientable by an external magnetic field. If M13 filaments are magnetically oriented during the library selection, their angular arrangement relative to the target surface would be changed, being expected to control the interference by the M13 main body. This study reports that the magnetic orientation of M13 filaments vertical to the target surface significantly affects the selection. When the target surface was affinitive to the M13 main body, this orientation notably suppressed the nonspecific adhesion. Furthermore, when the target surface was less affinitive to the M13 main body and intrinsically free from the nonspecific adhesion, this orientation drastically changed the population of M13 clones obtained through library selection. The method of using no chemicals but only a physical stimulus is simple, clean, and expected to expand the scope of phage display selection.

  • Access to Document (DOI)

• The sialyl-Tn antigen synthase genes regulates migration–proliferation dichotomy in prostate cancer cells under hypoxia

  Yamamoto D., Hongo H., Kosaka T., Aoki N., Oya M., Sato T.

  Glycoconjugate Journal （Glycoconjugate Journal)  40 （ 2 ） 199 - 212 2023.04

  ISSN  02820080

  　View Summary

  A low-oxygen (hypoxia) tumor microenvironment can facilitate chemotherapy and radiation therapy resistance in tumors and is associated with a poor prognosis. Hypoxia also affects PCa (prostate cancer) phenotype transformation and causes therapeutic resistance. Although O-glycans are known to be involved in the malignancy of various cancers under hypoxia, the expression and function of O-glycans in PCa are not well understood. In this study, the saccharide primer method was employed to analyze O-glycan expression in PCa cells. Results showed that the expression of sTn antigens was increased in PCa cells under hypoxia. Furthermore, it was found that ST6GalNAc1, the sTn antigen synthase gene, was involved in the migration–proliferation dichotomy and drug resistance in PCa cells under hypoxia. The results of this study will contribute to the development of novel diagnostic markers and drug targets for PCa under hypoxia.

  • Access to Document (DOI)

• Endocytosis-Like Vesicle Fission Mediated by a Membrane-Expanding Molecular Machine Enables Virus Encapsulation for In Vivo Delivery

  Uchida N., Ryu Y., Takagi Y., Yoshizawa K., Suzuki K., Anraku Y., Ajioka I., Shimokawa N., Takagi M., Hoshino N., Akutagawa T., Matsubara T., Sato T., Higuchi Y., Ito H., Morita M., Muraoka T.

  Journal of the American Chemical Society （Journal of the American Chemical Society)  145 （ 11 ） 6210 - 6220 2023.03

  ISSN  00027863

  　View Summary

  Biological membranes are functionalized by membrane-associated protein machinery. Membrane-associated transport processes, such as endocytosis, represent a fundamental and universal function mediated by membrane-deforming protein machines, by which small biomolecules and even micrometer-size substances can be transported via encapsulation into membrane vesicles. Although synthetic molecules that induce dynamic membrane deformation have been reported, a molecular approach enabling membrane transport in which membrane deformation is coupled with substance binding and transport remains critically lacking. Here, we developed an amphiphilic molecular machine containing a photoresponsive diazocine core (AzoMEx) that localizes in a phospholipid membrane. Upon photoirradiation, AzoMEx expands the liposomal membrane to bias vesicles toward outside-in fission in the membrane deformation process. Cargo components, including micrometer-size M13 bacteriophages that interact with AzoMEx, are efficiently incorporated into the vesicles through the outside-in fission. Encapsulated M13 bacteriophages are transiently protected from the external environment and therefore retain biological activity during distribution throughout the body via the blood following administration. This research developed a molecular approach using synthetic molecular machinery for membrane functionalization to transport micrometer-size substances and objects via vesicle encapsulation. The molecular design demonstrated in this study to expand the membrane for deformation and binding to a cargo component can lead to the development of drug delivery materials and chemical tools for controlling cellular activities.

  • Access to Document (DOI)

• Dihydromaniwamycin E, a Heat-Shock Metabolite from Thermotolerant Streptomyces sp. JA74, Exhibiting Antiviral Activity against Influenza and SARS-CoV-2 Viruses

  Saito S., Funayama K., Kato W., Okuda M., Kawamoto M., Matsubara T., Sato T., Sato A., Otsuguro S., Sasaki M., Orba Y., Sawa H., Maenaka K., Shindo K., Imoto M., Arai M.A.

  Journal of Natural Products （Journal of Natural Products)  85 （ 11 ） 2583 - 2591 2022.11

  ISSN  01633864

  　View Summary

  Dihydromaniwamycin E (1), a new maniwamycin derivative featuring an azoxy moiety, has been isolated from the culture extract of thermotolerant Streptomyces sp. JA74 along with the known analogue maniwamycin E (2). Compound 1 is produced only by cultivation of strain JA74 at 45 °C, and this type of compound has been previously designated a "heat shock metabolite (HSM)" by our research group. Compound 2 is detected as a production-enhanced metabolite at high temperature. Structures of 1 and 2 are elucidated by NMR and MS spectroscopic analyses. The absolute structure of 1 is determined after the total synthesis of four stereoisomers. Though the absolute structure of 2 has been proposed to be the same as the structure of maniwamycin D, the NMR and the optical rotation value of 2 are in agreement with those of maniwamycin E. Therefore, this study proposes a structural revision of maniwamycins D and E. Compounds 1 and 2 show inhibitory activity against the influenza (H1N1) virus infection of MDCK cells, demonstrating IC50values of 25.7 and 63.2 μM, respectively. Notably, 1 and 2 display antiviral activity against SARS-CoV-2, the causative agent of COVID-19, when used to infect 293TA and VeroE6T cells, with 1 and 2 showing IC50values (for infection of 293TA cells) of 19.7 and 9.7 μM, respectively. The two compounds do not exhibit cytotoxicity in these cell lines at those IC50concentrations.

  • Access to Document (DOI)

• Effective Cell Transfection in An Ultrasonically Levitated Droplet for Sustainable Technology

  Arai T., Sato T., Matsubara T.

  Advanced Science （Advanced Science)  9 （ 30 ）  2022.10

  　View Summary

  The levitation methodology, which enables us to operate a contactless reaction without a container, is likely to be a revolutionary technology in the fields of chemistry and biology to reduce the plastic waste in life science laboratories. Here, the authors show that plasmid DNA can be effectively transfected into animal cells in a floating droplet of culture medium levitated using ultrasonic standing waves. The data indicate that there is no significant damage to the plasmid and cells during the levitating transfection time, and the transgene expression efficiency and cellular uptake in the droplet are significantly higher than those in the conventional tube, with and without shaking. These results suggest the consolidation of the endocytic uptake pathway into macropinocytosis, indicating that ultrasonic levitation induced a change in cell characteristics. This study suggests that transfection methodology using ultrasonic levitation has the potential to advance the current experimental procedures in the field of cell engineering, in addition to presenting a revolutionary containerless reactor for sustainable technology.

  • Access to Document (DOI)

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Papers, etc., Registered in KOARA 【 Display / hide 】

Papers, etc., Registered in KOARA 【 Display / hide 】

• Development of diagnostic probes for mucopolysaccharidosis based on saccharide primer method

  Satō, Toshinori

  科学研究費補助金研究成果報告書 2018

• Analysis and control of cell function based on novel glycomics using saccharide primer method

  Sato, Toshinori

  科学研究費補助金研究成果報告書 2015

• Molecular evolution and functional analysis of influenza virus-binding peptides

  Sato, Toshinori

  科学研究費補助金研究成果報告書 2015

• Development of novel drug delivery system using ganglioside-binding peptides

  Sato, Toshinori

  科学研究費補助金研究成果報告書 2013

• Comparative glycomics toward the analysis of molecular mechanism of tumor metastasis using saccharide primer method

  Sato, Toshinori

  科学研究費補助金研究成果報告書 2011

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Reviews, Commentaries, etc. 【 Display / hide 】

Reviews, Commentaries, etc. 【 Display / hide 】

• 糖鎖プライマー法によるバイオコンビナトリアル合成

  佐藤 智典

  中分子創薬に資するペプチド・核酸・糖鎖の合成技術 (シーエムシー出版)     241 - 247 2018

  Article, review, commentary, editorial, etc. (other)

• 糖鎖模倣ペプチドによる抗インフルエンザ薬の研究

  松原 輝彦、佐藤　智典

  ペプチド医薬のスクリーニング・安定化・製剤化技術 (（株）技術情報協会)     221 - 228 2017

  Article, review, commentary, editorial, etc. (other)

• キトサンを用いたナノ粒子による遺伝子デリバリーシステム

  SATO TOSHINORI

  キチン・キトサンの最新科学技術、日本キチン・キトサン学会編 (博報堂出版)     209 - 226 2016

  Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media), Single Work

• キトサン

  佐藤 智典

  DDSキャリア作製プロトコル集 (シーエムシー出版)     109 - 117 2015

  Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media), Single Work

• 糖鎖プライマー法

  佐藤 智典

  糖鎖の新機能開発・応用ハンドブック (NTS)     380 - 382 2015

  Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media), Single Work

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Research Projects of Competitive Funds, etc. 【 Display / hide 】

Research Projects of Competitive Funds, etc. 【 Display / hide 】

• ウイルス変異体での細胞感染性に寄与する受容体糖鎖の解明

  2022.06

  -

  2024.03

  MEXT,JSPS, Grant-in-Aid for Scientific Research, 挑戦的研究（萌芽）, Principal investigator

• 感染症サーベイランスのためのインフルエンザウイルス新規検出法の創出

  2020.07

  -

  2022.03

  MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Challenging Research (Exploratory), Principal investigator

• 糖鎖プライマー法を基盤とするムコ多糖症診断プローブの創製

  2017.06

  -

  2019.03

  MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Challenging Research (Exploratory) , Principal investigator

Awards 【 Display / hide 】

Awards 【 Display / hide 】

• バイオ先端知賞

  2010.03, バイオビジネスコンペJAPAN実行委員会

• バイオビジネスコンペJAPANバイオ先端知賞

  SATO TOSHINORI, 2010.02, バイオビジネスコンペJAPAN実行委員会

  Type of Award： Award from publisher, newspaper, foundation, etc.

• FCCA奨励賞

  SATO TOSHINORI, 1995.03, FCCA

  Type of Award： Award from Japanese society, conference, symposium, etc.

• 日本化学会進歩賞

  SATO TOSHINORI, 1993.03, 日本化学会

  Type of Award： Award from Japanese society, conference, symposium, etc.

• 日本化学会第７回若い世代の特別講演会賞

  SATO TOSHINORI, 1991.10, 日本化学会

  Type of Award： Award from Japanese society, conference, symposium, etc.

Courses Taught 【 Display / hide 】

Courses Taught 【 Display / hide 】

• BACHELOR'S THESIS

  2023

• ADVANCED SCIENCES FOR DRUG DISCOVERY

  2023

• ADVANCED LABORATORY COURSE IN BIOSCIENCES AND INFORMATICS D

  2023

• ADVANCED LABORATORY COURSE IN BIOSCIENCES AND INFORMATICS C

  2023

• ADVANCED BIOMOLECULAR FUNCTION

  2023

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Memberships in Academic Societies 【 Display / hide 】

Memberships in Academic Societies 【 Display / hide 】

• 日本核酸医薬学会, 

  2015

  -

  Present

• 日本化学会フロンティア生命化学研究会, 

  2008.03

  -

  Present

• 日本再生医療学会, 

  2001.05

  -

  Present

• 東京糖鎖研究会, 

  2000

  -

  Present

• 遺伝子・デリバリー研究会, 

  2000

  -

  Present

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Committee Experiences 【 Display / hide 】

Committee Experiences 【 Display / hide 】

• 2017.03

  -

  2020.02

  会長, 日本化学会フロンティア生命化学研究会

• 2014.04

  -

  2016.03

  運営委員長, 高分子学会バイオ・高分子研究会

• 2014.03

  -

  2016.02

  主査, 日本化学会生体機能関連化学・バイオテクノロジーディビジョン

• 2010.01

  -

  Present

  副会長, 遺伝子・デリバリー研究会

• 2008.03

  -

  Present

  理事, 日本化学会フロンティア生命化学研究会

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