Soga, Tomoyoshi

写真a

Affiliation

Graduate School of Media and Governance （Shonan Fujisawa）

Position

Professor

Related Websites

External Links
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Message from the Faculty Member 【 Display / hide 】

  • 自分のこれまでの経験では、実験がうまくいってもそこから得られるものは何もありません。失敗して、原因をあれこれ考えることで自分が知らなかった知見を得たり、新しい発見をしたりします。したがって、多くのことに果敢にチェレンジしてたくさんの失敗を重ねて欲しいと思います。失敗が必ず皆さんの糧になります。

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Other Disclosed Information 【 Display / hide 】

  • Metabolomics, Analytical Chemistry

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Career 【 Display / hide 】

  • 1984.04
    -
    1992.03

    Application Chemist, Yokogawa Corp.

  • 1992.04
    -
    2001.03

    Yokogawa Analytical Systems Inc.

  • 2001.04
    -
    2002.03

    University of the Ryukyus, Visiting Professor

  • 2001.04
    -
    2005.03

    Faculty of Environmental Information/ Institute for Advanced Biosciences, Associate Professor

  • 2003.07
    -
    2010.03

    Human Metabolome Technologies Inc., Director

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Academic Background 【 Display / hide 】

  • 1980.04
    -
    1984.03

    Keio University, Faculty of Engineering, Applied Chemistry

    University, Graduated

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Academic Degrees 【 Display / hide 】

  • Ph.D., Toyohashi University of Technology, Dissertation, 2000.03

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Research Themes 【 Display / hide 】

  • 枯草菌、大腸菌、酵母等のバクテリアからイネやマウスの組織、ヒトの血液、尿、赤血球、ガン細胞等のあらゆる生物種の細胞内全代謝物質（メタボローム）の測定法, 

    　

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Books 【 Display / hide 】

  • Amino Acid Analysis : Methods and Protocols

    Alterman M.Ed., (Hirayama, A., Ikeda, S., Sato, A., Soga, T., contribution for Chapter 23）, Humana Press, 2019.07

    Scope: Chapter 23: Amino Acid Analysis by Capillary Electrophoresis-Mass Spectrometry,  Contact page： 307-313

    　View Summary

    Capillary electrophoresis-mass spectrometry (CE-MS) has been developed as a powerful tool in the analysis of charged compounds. To simultaneously analyze free amino acids, an electrolyte with low pH was used to positively charge all of the amino acids. In this condition, all protonated amino acids migrated toward the cathode in CE and then were sensitively and selectively detected by MS. This method is simple, rapid, and selective and could readily be applied to the analysis of free amino acids in various samples. In this chapter, the detailed procedure to analyze amino acids using CE-tandem mass spectrometry (MS/MS) is described.

  • Oceanography Challenges to Future Earth : Human and Natural Impacts on our Seas

    Komatsu, T., Ceccaldi, H-J., Yoshida, J., Prouzet, P., Henocque, Y. Ed., (Nakano, T., Shirakawa, H., Yeo, G., Devlin, R.H., Soga, T., contribution for Part IV ), Springer, 2019.02,  Page： 430

    Scope: Part IV Innovative Research: Metabolome profiling of growth hormone transgenic coho salmon by capillary electrophoresis time-of-flight mass spectrometry,  Contact page： 223-234

  • Capillary Electrophoresis-Mass Spectrometry for Metabolomics

    Ramautar R. Ed., (Hirayama A, Soga T. contribution for CHAPTER 7), The Royal Society of Chemistry, 2018.07,  Page： 300

    Scope: CHAPTER 7: CE-MS for anionic and cationic metabolic profiling: system optimization and applications,  Contact page： 134-160

  • ONCO-METABOLOMICS; A NEW CLUE TO UNDERSTAND CARCINOGENESIS, CANCER BIOLOGY AND TO DEVELOP NOVEL DIAGNOSTICS AND THERAPEUTICS

    Esmi, H., Mak, T.W., Soga, T., Suematsu, M.,Mori, M. Ed, Princess Takamatsu Cancer Research Fund, 2016.04

  • Metabolomics: Methods and Protocols

    Bjerrum, J. T. Ed. (Wakayama, M., Hirayama, A., Soga, T., contribution for Chapter 13), Humana Press, 2015.04,  Page： 269

    Scope: Chapter 13: Capillary Electrophoresis-Mass Spectrometry,  Contact page： 113-122

    　View Summary

    Capillary electrophoresis-mass spectrometry (CE-MS) has proven to be useful for metabolomics studies. Charged metabolites are first separated by CE based on charge and size and are subsequently selectively detected using MS. The major advantages of CE-MS are its high resolution and the fact that almost any charged species can be analyzed by two methods, both cationic and anionic. This technique can readily be applied to various types of biological samples originating from bacteria, plants, mammals, and body fluids. This chapter highlights detailed practical procedures for using this technology.

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Papers 【 Display / hide 】

  • Fusobacterium species are distinctly associated with patients with Lynch Syndrome colorectal cancer

    Salim, F., Mizutani, S., Shiba, S., Takamaru, H., Yamada, M., Nakajima, T., Yachida, T., Soga, T., Saito, Y., Fukuda, S., Yachida, S., Yamada, T.

    iScience 27 （ 7 ） 110181 - 110181 2024.07

    Research paper (scientific journal), Joint Work, Accepted

    　View Summary

    Accumulating evidence demonstrates clear correlation between the gut microbiota and sporadic colorectal cancer (CRC). Despite this, there is limited understanding of the association between the gut microbiota and CRC in Lynch Syndrome (LS), a hereditary type of CRC. Here, we analyzed fecal shotgun metagenomic and targeted metabolomic of 71 Japanese LS subjects. A previously published Japanese sporadic CRC cohort, which includes non-LS controls, was utilized as a non-LS cohort (n = 437). LS subjects exhibited reduced microbial diversity and low-Faecalibacterium enterotypes compared to non-LS. Patients with LS-CRC had higher levels of Fusobacterium nucleatum and fap2. Differential fecal metabolites and functional genes suggest heightened degradation of lysine and arginine in LS-CRC. A comparison between LS and non-LS subjects prior to adenoma formation revealed distinct fecal metabolites of LS subjects. These findings suggest that the gut microbiota plays a more responsive role in CRC tumorigenesis in patients with LS than those without LS.

  • Association of Nonalcoholic Fatty Liver Disease with Arterial Stiffness and its Metabolomic Profiling in Japanese Community-Dwellers

    Hirata, A., Harada, S., Iida, M., Kurihara, A., Fukai, K., Kuwabara, K., Kato, S., Matsumoto, M., Sata, M., Miyagawa, N., Toki, R., Edagawa, S., Sugiyama, D., Sato, A., Hirayama, A., Sugimoto, M., Soga, T., Tomita, M., Okamura, T., Takebayashi, T.

    J. Atheroscler. Thromb. 31 （ 7 ） 1031 - 1047 2024.07

    Research paper (scientific journal), Joint Work, Accepted

    　View Summary

    Aims: Nonalcoholic fatty liver disease (NAFLD) is known to be associated with atherosclerosis. This study focused on upstream changes in the process by which NAFLD leads to atherosclerosis. The study aimed to confirm the association between NAFLD and the cardio-ankle vascular index (CAVI), an indicator of subclinical atherosclerosis, and explore metabolites involved in both by assessing 94 plasma polar metabolites.

    Methods: A total of 928 Japanese community-dwellers (306 men and 622 women) were included in this study. The association between NAFLD and CAVI was examined using a multivariable regression model adjusted for confounders. Metabolites commonly associated with NAFLD and CAVI were investigated using linear mixed-effects models in which batch numbers of metabolite measurements were used as a random-effects variable, and false discovery rate-adjusted p-values were calculated. To determine the extent to which these metabolites mediated the association between NAFLD and CAVI, mediation analysis was conducted.

    Results: NAFLD was positively associated with CAVI (coefficients [95% Confidence intervals (CI)]=0.23 [0.09-0.37]; p=0.001). A total of 10 metabolites were involved in NAFLD and CAVI, namely, branched-chain amino acids (BCAAs; valine, leucine, and isoleucine), aromatic amino acids (AAAs; tyrosine and tryptophan), alanine, proline, glutamic acid, glycerophosphorylcholine, and 4-methyl-2-oxopentanoate. Mediation analysis showed that BCAAs mediated more than 20% of the total effect in the association between NAFLD and CAVI.

    Conclusions: NAFLD was associated with a marker of atherosclerosis, and several metabolites related to insulin resistance, including BCAAs and AAAs, could be involved in the process by which NAFLD leads to atherosclerosis.

    Keywords: Atherosclerosis; Branched-chain amino acids; Cardio-ankle vascular index; Insulin resistance; Metabolites; Nonalcoholic fatty liver disease.

  • The γ-glutamyl cycle serves as an amino acids supply system in colorectal cancer organoids under chronic hypoxia

    Tabata, S., Endo, H., Makinoshima, H., Soga, T., Inoue, M.

    Biochem. Biophys. Res. Commun. （Biochemical and Biophysical Research Communications)  714   149977 - 149977 2024.06

    Research paper (scientific journal), Joint Work, Accepted,  ISSN  0006291X

    　View Summary

    Malignant tumors are characterized by a hypoxic microenvironment, and metabolic reprogramming is necessary to ensure energy production and oxidative stress resistance. Although the microenvironmental properties of tumors vary under acute and chronic hypoxia, studies on chronic hypoxia-induced metabolic changes are limited. In the present study, we performed a comprehensive metabolic analysis in a chronic hypoxia model using colorectal cancer (CRC) organoids, and identified an amino acid supply system through the γ-glutamyl cycle, a glutathione recycling pathway. We analyzed the metabolic changes caused by hypoxia over time and observed that chronic hypoxia resulted in an increase in 5-oxoproline and a decrease in oxidized glutathione (GSSG) compared to acute hypoxia. These findings suggest that chronic hypoxia induces metabolic changes in the γ-glutamyl cycle. Moreover, inhibition of the γ-glutamyl cycle via γ-glutamyl cyclotransferase (GGCT) and γ-glutamyl transferase 1 (GGT1) knockdown significantly reversed chronic hypoxia-induced upregulation of 5-oxoproline and several amino acids. Notably, GGT1 knockdown downregulated the intracellular levels of γ-glutamyl amino acids. Conclusively, these results indicate that the γ-glutamyl cycle serves as an amino acid supply system in CRC under chronic hypoxia, which provides fresh insight into cancer metabolism under chronic hypoxia.

  • Molecular subtypes of lung adenocarcinoma present distinct immune tumor microenvironments

    Fukuda, H., Arai, K., Mizuno, H., Nishito, Y., Motoi, N., Arai, Y., Hiraoka, N., Shibata, T., Sonobe, Y., Kayukawa, Y., Hashimoto, E., Takahashi, M., Fujii, E., Maruyama, T., Kuwabara, K., Nishizawa, T., Mizoguchi, Y., Yoshida, Y., Watanabe, S., Yamashita, M., Kitano, S., Sakamoto, H., Nagata, Y., Mitsumori, R., Ozaki, K., Niida, S., Kanai, Y., Hirayama, A., Soga, T., Tsukada, K., Yabuki, N., Shimada, M., Kitazawa, T., Natori, O., Sawada, N., Kato, A., Yoshida, T., Yasuda, K., Ochiai, A., Tsunoda, H., Aoki, K

    Cancer Sci. （Cancer Science)  115 （ 6 ） 1763 - 1777 2024.06

    Research paper (scientific journal), Joint Work, Accepted,  ISSN  13479032

    　View Summary

    Overcoming resistance to immune checkpoint inhibitors is an important issue in patients with non-small-cell lung cancer (NSCLC). Transcriptome analysis shows that adenocarcinoma can be divided into three molecular subtypes: terminal respiratory unit (TRU), proximal proliferative (PP), and proximal inflammatory (PI), and squamous cell carcinoma (LUSQ) into four. However, the immunological characteristics of these subtypes are not fully understood. In this study, we investigated the immune landscape of NSCLC tissues in molecular subtypes using a multi-omics dataset, including tumor-infiltrating leukocytes (TILs) analyzed using flow cytometry, RNA sequences, whole exome sequences, metabolomic analysis, and clinicopathologic findings. In the PI subtype, the number of TILs increased and the immune response in the tumor microenvironment (TME) was activated, as indicated by high levels of tertiary lymphoid structures, and high cytotoxic marker levels. Patient prognosis was worse in the PP subtype than in other adenocarcinoma subtypes. Glucose transporter 1 (GLUT1) expression levels were upregulated and lactate accumulated in the TME of the PP subtype. This could lead to the formation of an immunosuppressive TME, including the inactivation of antigen-presenting cells. The TRU subtype had low biological malignancy and "cold" tumor-immune phenotypes. Squamous cell carcinoma (LUSQ) did not show distinct immunological characteristics in its respective subtypes. Elucidation of the immune characteristics of molecular subtypes could lead to the development of personalized immune therapy for lung cancer. Immune checkpoint inhibitors could be an effective treatment for the PI subtype. Glycolysis is a potential target for converting an immunosuppressive TME into an antitumorigenic TME in the PP subtype.

  • SGLT2 inhibition eliminates senescent cells and alleviates pathological aging

    Katsuumi, G., Shimizu, I., Suda, M., Yoshida, Y., Furihata, T., Joki, Y., Hsiao, CL., Jiaqi, L., Fujiki, S., Abe, M., Sugimoto, M., Soga, T., Minamino, T

    Nat. Aging online 2024.05

    Research paper (scientific journal), Joint Work

    　View Summary

    It has been reported that accumulation of senescent cells in various tissues contributes to pathological aging and that elimination of senescent cells (senolysis) improves age-associated pathologies. Here, we demonstrate that inhibition of sodium-glucose co-transporter 2 (SGLT2) enhances clearance of senescent cells, thereby ameliorating age-associated phenotypic changes. In a mouse model of dietary obesity, short-term treatment with the SGLT2 inhibitor canagliflozin reduced the senescence load in visceral adipose tissue and improved adipose tissue inflammation and metabolic dysfunction, but normalization of plasma glucose by insulin treatment had no effect on senescent cells. Canagliflozin extended the lifespan of mice with premature aging even when treatment was started in middle age. Metabolomic analyses revealed that short-term treatment with canagliflozin upregulated 5-aminoimidazole-4-carboxamide-1-β-D-ribofuranoside, enhancing immune-mediated clearance of senescent cells by downregulating expression of programmed cell death-ligand 1. These findings suggest that inhibition of SGLT2 has an indirect senolytic effect by enhancing endogenous immunosurveillance of senescent cells.

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Papers, etc., Registered in KOARA 【 Display / hide 】

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Reviews, Commentaries, etc. 【 Display / hide 】

  • 腫瘍における分岐鎖アミノ酸トランスポーターの役割

    Saito, Y., Soga, T.

    Experimental Medicine (YODOSHA)  40 ( 14 ) 2239 - 2244 2022.08

    Article, review, commentary, editorial, etc. (other), Joint Work

  • アミノ酸トランスポーターを標的とした個別化医療

    Precision Medicine 4 ( 13 ) 18 - 22 2021.11

    Article, review, commentary, editorial, etc. (other), Joint Work

  • メタボロミクスによるがん幹細胞の代謝研究

    北島正二朗、曽我朋義

    （別冊 医学のあゆみ）　治療標的としてのがん幹細胞 (Ishiyaku Pub,Inc.)     117 - 121 2021.03

    Article, review, commentary, editorial, etc. (other), Joint Work

  • メタボローム解析に基づく癌診断法の開発

    Soga, T., Sugimoto, M.

    Gastroenterology & Hepatology (Kagakuhyoronsya Co., Ltd.)  8 ( 2 ) 137 - 143 2020.08

    Joint Work

  • はじめに-メタボローム解析UPDATE

    Soga, T.

    （別冊・医学のあゆみ） メタボローム解析UPDATE (Ishiyaku Pub,Inc.)   2020.06

    Single Work

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Presentations 【 Display / hide 】

  • Development of CE-MS Metabolomics and its Application in Cancer

    Tomoyoshi Soga

    Metabolomics2024, 20th Annual Confereence of the Metabolomics Society (Osaka) , 

    2024.06

  • Multi Omics analysis of colorectal cancer metabolism

    2018 International Meeting on 22nd MDO and 33rd JSSX (Ishikawa Ongakudo, Kanazawa, Ishikawa) , 

    2018.10
    , 

    Oral presentation (invited, special), JSSX (The Japanese Society for the Study of Xenobiotics)、MDO(Microsomes and Drug Oxidations)

  • Malti-omics reveals MYC as a master regulator of colorectal cancer metabolism

    SOGA TOMOYOSHI

    The 1st International Symposium for Trans-Omics (Koshiba Hall, The University of Tokyo, Hongo Campus) , 

    2017.11
    , 

    Oral presentation (invited, special)

  • Onco-metabolites and cancer specific metabolic pathways

    SOGA TOMOYOSHI

    American Association for Cancer Research Annual Meeting 2017, AACR2017, 

    2017.04
    , 

    Oral presentation (invited, special)

  • What Causes Altered Metabolism in Colon Cancer Cells

    SOGA TOMOYOSHI

    46th International Symposium of The Princess Takamatsu Cancer Research Found (Palece Hotel Tokyo, Japan) , 

    2015.11
    , 

    Oral presentation (invited, special)

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Research Projects of Competitive Funds, etc. 【 Display / hide 】

  • Study of therapeutic approaches to SUCLA2-deleted cancers

    2024.05
    -
    2025.03

    Japan Agency for Medical Research and Development(AMED), Project for Promotion of Cancer Research and Therapeutic Evolution（P-PROMOTE）, Takahashi, Chiaki, Commissioned ｒesearch, Coinvestigator(s)

  • Transomic analysis of hybernation and torpor Planned Research

    2023.06
    -
    2025.03

    Ministry of Education,Culture,Sports,Science and Technology(MEXT)/Japan Society for the Promotion of Science(JSPS) , Grant-in-Aid for Transformative Research Areas (A), Kuroda, Shinya, Research grant, Coinvestigator(s)

  • 大腸がんにおけるオンコメタボライト・L-2HGの分子基盤の解明

    2022.06
    -
    2025.03

    Ministry of Education,Culture,Sports,Science and Technology(MEXT)/Japan Society for the Promotion of Science(JSPS) , Grant-in-Aid for Scientific Research (C), Tabata, Sho, Other, Coinvestigator(s)

  • Spatio-temporal trans-omics analysis of metabolic control mechanisms of multi-cellular organ systems

    2021.10
    -
    2025.03

    Japan Science and Technology Agency(JST), Strategic Basic Research Programs CREST, Kuroda, Shinya, Commissioned ｒesearch, Coinvestigator(s)

  • Development of novel therapy targeting SUCLA2 deficiency in advanced prostate cancer

    2021.05
    -
    2023.03

    Japan Agency for Medical Research and Development(AMED), Project for Cancer Research and Therapeutic Evolution (P-CREATE) , Takahashi, C., Commissioned ｒesearch, Coinvestigator(s)

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Works 【 Display / hide 】

  • 経済産業省主催「バイオ人材育成事業」メタボローム実習講師

    SOGA TOMOYOSHI

    2004.12
    , 

    Other, Joint

  • 日経BP社主催バイオファイナンスギルド　メタボローム講座

    SOGA TOMOYOSHI

    2004.08
    , 

    Other, Joint

  • キャピラリー電気泳動による無機陰イオン、有機酸、アミノ酸の分析

    そがともよし

    2001.10
    -
    Present
    , 

    Other

    　View Details

    キャピラリー電気泳動法の装置、測定法の原理および様々な測定例を解説した

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Intellectual Property Rights, etc. 【 Display / hide 】

  • カテコールアミン類の分析方法(Apparatus and Method for Catecholamine Analysis)

    Date applied： 特願平01-272206  1989.10 

    Date announced： 特開平03-134561  1991.06 

    Date issued： 特許第2833058号  1998.10

    Patent, Single

  • 陰イオン性化合物の分析方法(Apparatus and Method for Anion Analysis)

    Date applied： 特願平08-143048  1996.06 

    Date announced： 特開平09-325130  1997.12 

    Date issued： 特許第2912232号  1999.04

    Patent, Single

  • キャピラリー電気泳動による陰イオン、アミノ酸、糖類の分析方法及び装置(Capillary Electrophoresis Apparatus and Method for Anions, Amino Acids and Carbohydrate Analysis)

    Date applied： 特願平10-145244  1998.05 

    Date announced： 特開平11-337524  1999.12 

    Date issued： 特許第3038184号  2000.02

    Patent, Single

  • 陰イオン性化合物の分離分析方法及び装置(Apparatus and Method for Anion Analysis)

    Date applied： 特願2001-224341  2001.07 

    Date announced： 特開2003-035698  2003.02 

    Date issued： 特許第3341765号  2002.08

    Patent, Single

  • 電気泳動測定によるイオン性化合物の移動時間予想方法

    Date applied： 特願2004-245728  2004.08 

    Date announced： 特開2006-064472  2006.03 

    Date issued： 特許第3871689号  2006.10

    Patent, Joint

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Awards 【 Display / hide 】

  • Fukuzawa Award

    Soga, T., 2022.11, Keio Univ., メタボローム（細胞内全代謝物質）解析技術の開発と実用化

    Type of Award： Keio commendation etc.

  • The 2nd Shigeru Terabe Award

    2015.11,  Division of Electrophoresis, Japan Society for Analytical Chemistry, CE-MSメタボローム測定技術の開発と実用化

    Type of Award： Award from Japanese society, conference, symposium, etc.

  • Keio Award

    SOGA Tomoyoshi, 2011.11, Keio University, CE-MSメタボローム測定技術の開発と実用化

    Type of Award： Keio commendation etc.

  • 第7回酸化ストレスと肝研究会　奨励賞

    SOGA Tomoyoshi, 2010.11, 酸化ストレスと肝研究会, メタボロミクスによる新規酸化ストレスマーカーの同定と肝臓疾患スクリーニング

    Type of Award： Award from Japanese society, conference, symposium, etc.

    　View Description

    第7回酸化ストレスと肝研究会における研究発表による

  • The prize of the chairman of HATSUMEI KYOKAI

    SOGA Tomoyoshi, 2009.07, Japan Institute of Invention and Innovation, Apparatus and Method for Metabolome Analysis

    Type of Award： Other

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Courses Taught 【 Display / hide 】

  • ADVANCED RESEARCH(SYSTEMS BIOLOGY)

    2024

  • ACADEMIC PROJECT(ADVANCED BIOSCIENCES B)

    2024

  • ACADEMIC PROJECT(ADVANCED BIOSCIENCES A)

    2024

  • GRADUATION PROJECT 1

    2024

  • SEMINAR B

    2024

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