曽我 朋義 (ソガ トモヨシ)

Soga, Tomoyoshi



政策・メディア研究科 (湘南藤沢)





教員からのメッセージ 【 表示 / 非表示

  • 自分のこれまでの経験では、実験がうまくいってもそこから得られるものは何もありません。失敗して、原因をあれこれ考えることで自分が知らなかった知見を得たり、新しい発見をしたりします。したがって、多くのことに果敢にチェレンジしてたくさんの失敗を重ねて欲しいと思います。失敗が必ず皆さんの糧になります。

その他公開情報 【 表示 / 非表示

  • メタボロミクス、分析化学

経歴 【 表示 / 非表示

  • 1984年04月


  • 1992年04月


  • 2001年04月

    琉球大学 , 客員教授

  • 2001年04月

    慶應義塾大学環境情報学部/先端生命科学研究所, 助教授(有期)

  • 2003年07月

    ヒューマン・メタボローム・テクノロジーズ株式会社, 取締役

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学歴 【 表示 / 非表示

  • 1980年04月

    慶應義塾大学, 工学部, 応用化学科

    大学, 卒業

学位 【 表示 / 非表示

  • 工学博士, 豊橋技術科学大学, 論文, 2000年03月


研究テーマ 【 表示 / 非表示

  • 枯草菌、大腸菌、酵母等のバクテリアからイネやマウスの組織、ヒトの血液、尿、赤血球、ガン細胞等のあらゆる生物種の細胞内全代謝物質(メタボローム)の測定法, 



著書 【 表示 / 非表示

  • Amino Acid Analysis : Methods and Protocols

    Alterman M.Ed., (Hirayama, A., Ikeda, S., Sato, A., Soga, T., contribution for Chapter 23), Humana Press, 2019年07月

    担当範囲: Chapter 23: Amino Acid Analysis by Capillary Electrophoresis-Mass Spectrometry,  担当ページ: 307-313


    Capillary electrophoresis-mass spectrometry (CE-MS) has been developed as a powerful tool in the analysis of charged compounds. To simultaneously analyze free amino acids, an electrolyte with low pH was used to positively charge all of the amino acids. In this condition, all protonated amino acids migrated toward the cathode in CE and then were sensitively and selectively detected by MS. This method is simple, rapid, and selective and could readily be applied to the analysis of free amino acids in various samples. In this chapter, the detailed procedure to analyze amino acids using CE-tandem mass spectrometry (MS/MS) is described.

  • Oceanography Challenges to Future Earth : Human and Natural Impacts on our Seas

    Komatsu, T., Ceccaldi, H-J., Yoshida, J., Prouzet, P., Henocque, Y. Ed., (Nakano, T., Shirakawa, H., Yeo, G., Devlin, R.H., Soga, T., contribution for Part IV ), Springer, 2019年02月,  ページ数: 430

    担当範囲: Part IV Innovative Research: Metabolome profiling of growth hormone transgenic coho salmon by capillary electrophoresis time-of-flight mass spectrometry,  担当ページ: 223-234

  • Capillary Electrophoresis-Mass Spectrometry for Metabolomics

    Ramautar R. Ed., (Hirayama A, Soga T. contribution for CHAPTER 7), The Royal Society of Chemistry, 2018年07月,  ページ数: 300

    担当範囲: CHAPTER 7: CE-MS for anionic and cationic metabolic profiling: system optimization and applications,  担当ページ: 134-160


    Esmi, H., Mak, T.W., Soga, T., Suematsu, M.,Mori, M. Ed, Princess Takamatsu Cancer Research Fund, 2016年04月

  • Metabolomics: Methods and Protocols

    Bjerrum, J. T. Ed. (Wakayama, M., Hirayama, A., Soga, T., contribution for Chapter 13), Humana Press, 2015年04月,  ページ数: 269

    担当範囲: Chapter 13: Capillary Electrophoresis-Mass Spectrometry,  担当ページ: 113-122


    Capillary electrophoresis-mass spectrometry (CE-MS) has proven to be useful for metabolomics studies. Charged metabolites are first separated by CE based on charge and size and are subsequently selectively detected using MS. The major advantages of CE-MS are its high resolution and the fact that almost any charged species can be analyzed by two methods, both cationic and anionic. This technique can readily be applied to various types of biological samples originating from bacteria, plants, mammals, and body fluids. This chapter highlights detailed practical procedures for using this technology.

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論文 【 表示 / 非表示

  • Serum Carbohydrate Antigen 19-9 and Metabolite Hypotaurine Are Predictive Markers for Early Recurrence of Pancreatic Ductal Adenocarcinoma

    Nagao, M., Oshima, M., Suto, H., Sugimoto, M., Enomoto, A., Murakami, T., Shimomura, A., Wada, Y., Matsukawa, H., Ando, Y., Kishino, T., Kumamoto, K., Kobara, H., Kamada, H., Masaki, T., Soga, T., Okano, K.

    Pancreas 53 ( 4 ) e301 - e309 2024年04月

    研究論文(学術雑誌), 共著, 査読有り


    Objective: A significant number of patients experience early recurrence after surgical resection for pancreatic ductal adenocarcinoma (PDAC), negating the benefit of surgery. The present study conducted clinicopathologic and metabolomic analyses to explore the factors associated with the early recurrence of PDAC.

    Materials and methods: Patients who underwent pancreatectomy for PDAC at Kagawa University Hospital between 2011 and 2020 were enrolled. Tissue samples of PDAC and nonneoplastic pancreas were collected and frozen immediately after resection. Charged metabolites were quantified by capillary electrophoresis-mass spectrometry. Patients who relapsed within 1 year were defined as the early recurrence group.

    Results: Frozen tumor tissue and nonneoplastic pancreas were collected from 79 patients. The clinicopathologic analysis identified 11 predictive factors, including preoperative carbohydrate antigen 19-9 levels. The metabolomic analysis revealed that only hypotaurine was a significant risk factor for early recurrence. A multivariate analysis, including clinical and metabolic factors, showed that carbohydrate antigen 19-9 and hypotaurine were independent risk factors for early recurrence (P = 0.045 and P = 0.049, respectively). The recurrence-free survival rate 1 year after surgery with both risk factors was only 25%.

    Conclusions: Our results suggested that tumor hypotaurine is a potential metabolite associated with early recurrence. Carbohydrate antigen 19-9 and hypotaurine showed a vital utility for predicting early recurrence.

  • Genetic mutation in Escherichia coli genome during adaptation to the murine intestine is optimized for the host diet

    Tsukimi, T., Obana, N., Shigemori, S., Arakawa, K., Miyauchi, E., Yang, J., Song, I., Ashino, Y., Wakayama, M., Soga, T., Tomita, M., Ohno, H., Mori, H., Fukuda, S.

    mSystems 9 ( 2 ) e0112323 - e0112323 2024年02月

    研究論文(学術雑誌), 共著, 査読有り


    The gut microbiota is closely associated with human health and is greatly impacted by the host diet. Bacteria such as Escherichia coli live in the gut all throughout the life of a human host and adapt to the intestinal environment. Adaptive mutations in E. coli are reported to enhance fitness in the mammalian intestine, but to what extent is still poorly known. It is also unknown whether the host diet affects what genes are mutated and to what extent fitness is affected. This study suggests that genetic mutations in the E. coli K-12 strain are selected in response to the intestinal environment and facilitate efficient utilization of abundant nutrients in the germ-free mouse intestine. Our study provides a better understanding of these intestinal adaptation mechanisms of gut microbes.

  • Association of Nonalcoholic Fatty Liver Disease with Arterial Stiffness and its Metabolomic Profiling in Japanese Community-Dwellers

    Hirata, A., Harada, S., Iida, M., Kurihara, A., Fukai, K., Kuwabara, K., Kato, S., Matsumoto, M., Sata, M., Miyagawa, N., Toki, R., Edagawa, S., Sugiyama, D., Sato, A., Hirayama, A., Sugimoto, M., Soga, T., Tomita, M., Okamura, T., Takebayashi, T.

    J. Atheroscler. Thromb. online 2024年02月

    研究論文(学術雑誌), 共著, 査読有り


    Aims: Nonalcoholic fatty liver disease (NAFLD) is known to be associated with atherosclerosis. This study focused on upstream changes in the process by which NAFLD leads to atherosclerosis. The study aimed to confirm the association between NAFLD and the cardio-ankle vascular index (CAVI), an indicator of subclinical atherosclerosis, and explore metabolites involved in both by assessing 94 plasma polar metabolites.

    Methods: A total of 928 Japanese community-dwellers (306 men and 622 women) were included in this study. The association between NAFLD and CAVI was examined using a multivariable regression model adjusted for confounders. Metabolites commonly associated with NAFLD and CAVI were investigated using linear mixed-effects models in which batch numbers of metabolite measurements were used as a random-effects variable, and false discovery rate-adjusted p-values were calculated. To determine the extent to which these metabolites mediated the association between NAFLD and CAVI, mediation analysis was conducted.

    Results: NAFLD was positively associated with CAVI (coefficients [95% Confidence intervals (CI)]=0.23 [0.09-0.37]; p=0.001). A total of 10 metabolites were involved in NAFLD and CAVI, namely, branched-chain amino acids (BCAAs; valine, leucine, and isoleucine), aromatic amino acids (AAAs; tyrosine and tryptophan), alanine, proline, glutamic acid, glycerophosphorylcholine, and 4-methyl-2-oxopentanoate. Mediation analysis showed that BCAAs mediated more than 20% of the total effect in the association between NAFLD and CAVI.

    Conclusions: NAFLD was associated with a marker of atherosclerosis, and several metabolites related to insulin resistance, including BCAAs and AAAs, could be involved in the process by which NAFLD leads to atherosclerosis.

    Keywords: Atherosclerosis; Branched-chain amino acids; Cardio-ankle vascular index; Insulin resistance; Metabolites; Nonalcoholic fatty liver disease.

  • Reliability of Time-Series Plasma Metabolome Data over 6 Years in a Large-Scale Cohort Study

    Miyake, A., Harada, S., Sugiyama, D., Matsumoto, M., Hirata, A., Miyagawa, N., Toki, R., Edagawa, S., Kuwabara, K., Okamura, T., Sato, A., Amano, K., Hirayama, A., Sugimoto, M., Soga, T., Tomita, M., Arakawa, K., Takebayashi, T., Iida, M.

    Metabolites  14 ( 1 ) 77 - 77 2024年01月

    研究論文(学術雑誌), 共著, 査読有り


    Studies examining long-term longitudinal metabolomic data and their reliability in large-scale populations are limited. Therefore, we aimed to evaluate the reliability of repeated measurements of plasma metabolites in a prospective cohort setting and to explore intra-individual concentration changes at three time points over a 6-year period. The study participants included 2999 individuals (1317 men and 1682 women) from the Tsuruoka Metabolomics Cohort Study, who participated in all three surveys-at baseline, 3 years, and 6 years. In each survey, 94 plasma metabolites were quantified for each individual and quality control (QC) sample. The coefficients of variation of QC, intraclass correlation coefficients, and change rates of QC were calculated for each metabolite, and their reliability was classified into three categories: excellent, fair to good, and poor. Seventy-six percent (71/94) of metabolites were classified as fair to good or better. Of the 39 metabolites grouped as excellent, 29 (74%) in men and 26 (67%) in women showed significant intra-individual changes over 6 years. Overall, our study demonstrated a high degree of reliability for repeated metabolome measurements. Many highly reliable metabolites showed significant changes over the 6-year period, suggesting that repeated longitudinal metabolome measurements are useful for epidemiological studies.

  • Metabolic Hallmarks for Purine Nucleotide Biosynthesis in Small-Cell Lung Carcinoma

    Tabata, S., Umemura, S., Narita, M., Udagawa, H., Ishikawa, T., Tsuboi, M., Goto, K., Ishii, G., Tsuchihara, K., Ochiai, A., Kobayashi, S., Soga, T., Makinoshima, H.

    Mol. Cancer Res. 22 ( 1 ) 82 - 93 2024年01月

    研究論文(学術雑誌), 共著, 査読有り


    Small-cell lung cancer (SCLC) has a poor prognosis, emphasizing the necessity for developing new therapies. The de novo synthesis pathway of purine nucleotides, which is involved in the malignant growth of SCLC, has emerged as a novel therapeutic target. Purine nucleotides are supplied by two pathways: de novo and salvage. However, the role of the salvage pathway in SCLC and the differences in utilization and crosstalk between the two pathways remain largely unclear. Here, we found that deletion of the HPRT1 gene, which codes for the rate-limiting enzyme of the purine salvage pathway, significantly suppressed tumor growth in vivo in several SCLC cells. We also demonstrated that HPRT1 expression confers resistance to lemetrexol (LMX), an inhibitor of the purine de novo pathway. Interestingly, HPRT1-knockout had less effect on SCLC SBC-5 cells, which are more sensitive to LMX than other SCLC cell lines, suggesting that a preference for either the purine de novo or salvage pathway occurs in SCLC. Furthermore, metabolome analysis of HPRT1-knockout cells revealed increased intermediates in the pentose phosphate pathway and elevated metabolic flux in the purine de novo pathway, indicating compensated metabolism between the de novo and salvage pathways in purine nucleotide biosynthesis. These results suggest that HPRT1 has therapeutic implications in SCLC and provide fundamental insights into the regulation of purine nucleotide biosynthesis.

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KOARA(リポジトリ)収録論文等 【 表示 / 非表示

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総説・解説等 【 表示 / 非表示

  • 腫瘍における分岐鎖アミノ酸トランスポーターの役割


    実験医学 (羊土社)  40 ( 14 ) 2239 - 2244 2022年08月

    記事・総説・解説・論説等(その他), 共著

  • アミノ酸トランスポーターを標的とした個別化医療

    Precision Medicine 4 ( 13 ) 18 - 22 2021年11月

    記事・総説・解説・論説等(その他), 共著

  • メタボロミクスによるがん幹細胞の代謝研究


    (別冊 医学のあゆみ) 治療標的としてのがん幹細胞 (医歯薬出版株式会社)     117 - 121 2021年03月

    記事・総説・解説・論説等(その他), 共著

  • メタボローム解析に基づく癌診断法の開発


    消化器・肝臓内科 (科学評論社)  8 ( 2 ) 137 - 143 2020年08月


  • はじめに-メタボローム解析UPDATE


    (別冊・医学のあゆみ) メタボローム解析UPDATE (医歯薬出版株式会社)   2020年06月


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研究発表 【 表示 / 非表示

  • Multi Omics analysis of colorectal cancer metabolism

    2018 International Meeting on 22nd MDO and 33rd JSSX (Ishikawa Ongakudo, Kanazawa, Ishikawa) , 


    口頭発表(招待・特別), JSSX (The Japanese Society for the Study of Xenobiotics)、MDO(Microsomes and Drug Oxidations)

  • Malti-omics reveals MYC as a master regulator of colorectal cancer metabolism

    曽我 朋義

    The 1st International Symposium for Trans-Omics (Koshiba Hall, The University of Tokyo, Hongo Campus) , 



  • Onco-metabolites and cancer specific metabolic pathways


    American Association for Cancer Research Annual Meeting 2017, AACR2017, 



  • What Causes Altered Metabolism in Colon Cancer Cells


    46th International Symposium of The Princess Takamatsu Cancer Research Found (Palece Hotel Tokyo, Japan) , 



  • CE-MS Metabolomics and Application to Cancer cell Metabolism


    11th International Conference of the Metabolomics Society, Metabolomics 2015, (Hyatt Regency San Francisco Airport, Burlingame, California, USA) , 



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競争的研究費の研究課題 【 表示 / 非表示

  • 時空間トランスオミクスを用いた多細胞・臓器連関代謝制御の解明


    国立研究開発法人科学技術振興機構 (JST),  戦略的創造研究推進事業 CREST, 黒田 真也, 受託研究,  研究分担者

  • SUCLA2遺伝子欠失を標的とする進行前立腺がんの新規治療法開発


    日本医療研究開発機構(AMED), 次世代がん医療創生研究事業 , 髙橋 智聡, 受託研究,  研究分担者

  • ミトコンドリア先制医療


    日本医療研究開発機構(AMED), ムーショット型研究開発事業, 阿部 高明, 受託研究,  研究分担者

  • SUCLA2遺伝子欠失によって生じる代謝脆弱性を標的とする新規がん治療法探索


    日本医療研究開発機構(AMED), 次世代がん医療創生研究事業(P-CREATE) , 髙橋 智聡, 受託研究,  研究分担者

  • メタボロミクスを用いたフレイル・認知機能低下に対する超早期リスク判別指標の開発


    文部科学省・日本学術振興会, 科学研究費助成事業 基盤研究(A), 武林 亨, 補助金,  研究分担者

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Works 【 表示 / 非表示

  • 経済産業省主催「バイオ人材育成事業」メタボローム実習講師

    曽我 朋義


    その他, 共同

  • 日経BP社主催バイオファイナンスギルド メタボローム講座

    曽我 朋義


    その他, 共同

  • キャピラリー電気泳動による無機陰イオン、有機酸、アミノ酸の分析






知的財産権等 【 表示 / 非表示

  • カテコールアミン類の分析方法

    出願日: 特願平01-272206  1989年10月 

    公開日: 特開平03-134561  1991年06月 

    発行日: 特許第2833058号  1998年10月

    特許権, 単独

  • 陰イオン性化合物の分析方法

    出願日: 特願平08-143048  1996年06月 

    公開日: 特開平09-325130  1997年12月 

    発行日: 特許第2912232号  1999年04月

    特許権, 単独

  • キャピラリー電気泳動による陰イオン、アミノ酸、糖類の分析方法及び装置

    出願日: 特願平10-145244  1998年05月 

    公開日: 特開平11-337524  1999年12月 

    発行日: 特許第3038184号  2000年02月

    特許権, 単独

  • 陰イオン性化合物の分離分析方法及び装置

    出願日: 特願2001-224341  2001年07月 

    公開日: 特開2003-035698  2003年02月 

    発行日: 特許第3341765号  2002年08月

    特許権, 単独

  • 電気泳動測定によるイオン性化合物の移動時間予想方法

    出願日: 特願2004-245728  2004年08月 

    公開日: 特開2006-064472  2006年03月 

    発行日: 特許第3871689号  2006年10月

    特許権, 共同

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受賞 【 表示 / 非表示

  • 福澤賞

    曽我 朋義, 2022年11月, 慶應義塾, メタボローム(細胞内全代謝物質)解析技術の開発と実用化

    受賞区分: 塾内表彰等

  • 第2回寺部茂賞

    2015年11月, 日本分析化学会 電気泳動分析研究懇談会, CE-MSメタボローム測定技術の開発と実用化

    受賞区分: 国内学会・会議・シンポジウム等の賞

  • 義塾賞

    曽我 朋義, 2011年11月, 慶應義塾大学, CE-MSメタボローム測定技術の開発と実用化

    受賞区分: 塾内表彰等

  • 第7回酸化ストレスと肝研究会 奨励賞

    曽我 朋義, 2010年11月, 酸化ストレスと肝研究会, メタボロミクスによる新規酸化ストレスマーカーの同定と肝臓疾患スクリーニング

    受賞区分: 国内学会・会議・シンポジウム等の賞



  • 平成21年度全国発明表彰 発明協会会長賞

    曽我 朋義, 2009年07月, 社団法人 発明協会, メタボローム測定装置の発明

    受賞区分: その他

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担当授業科目 【 表示 / 非表示

  • 研究会B


  • メタボロミクス


  • メタボローム解析実習


  • 修士研究会


  • 特別研究


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