Papers - Soga, Tomoyoshi

Establishment of Basic Lipidomics Platforms for Discovery of Lipid Biomarkers

Ikeda, K., Taguchi, R., Soga, T.

Bunseki Kagaku (The Japan Society for Analytical Chemistry)  61 ( 6 ) 501 - 512 2012.06

Research paper (scientific journal), Joint Work

　View Summary

Lipidomics is one of the major approaches in metabolomics, and often uses a liquid chromatography-mass spectrometer (LC-MS) to obtain profiling of lipid metabolites and to detect changing molecules statistically. However, previously reported methods have been technically insufficient in the simultaneous analysis of amphiphilic glycosphingolipids, such as gangliosides, and in the global detection of various polar metabolites from complex lipid mixtures. Here, we report on newly targeted and non-targeted methods to measure these lipid metabolites sensitively. In the targeted approach, we were able to detect individual ganglioside molecular species using the combination of reversed-phase or hydrophilic interaction liquid chromatography and ESI-MS with multiple reaction monitoring (MRM).
Meanwhile, in a non-targeted approach, global analysis of various polar metabolites was possible by reverse-phased high-resolution (LC) coupled quadrapole time-of-flight MS and sensitive twodimensional (2D) profiling. Our methods will be useful for the elucidation of cancers, metabolic syndrome and nervous-system diseases, etc. ; these diagnostic markers may be detectable.

• Access to Document (DOI)

Metabolome Analysis of Colon Tumor Tissues by Capillary Electrophoresis Time-of-Flight Mass Spectrometer

Hirayama, A., Kami, K., Sugimoto, M., Tomita, M., Soga, T.

J. Mass Spectrom. Soc. Jpn. 57 ( 3 ) 183 - 186 2009.06

Research paper (scientific journal), Joint Work

　View Summary

Capillary electrophoresis-mass spectrometry based metabolomics exhibits unlimited potentials to elucidate global metabolic profiles of various types of samples including clinical tissues. In addition, non-targeted metabolome analysis by this method holds the promise of discovering novel biomarker candidates. In this commentary, we introduce our strategies for studying cancer-specific metabolism by analyzing metabolome of surgically excised tumor tissues.

• Access to Document (DOI)

CE-MSによるメタボローム解析

Soga, T.

生物物理化学 52 ( 3 ) 91 - 94 2008.09

Research paper (scientific journal), Single Work

　View Summary

Metabolomics, which can be defined as the measurement of the level of all intracellular metabolites, has become a powerful new tool for gaining insight into cellular and physiological responses. Here we have developed a comprehensive and quantitative analysis method based on capillary electrophoresis mass spectrometry (CE-MS). In this marriage of techniques, CE confers rapid analysis and efficient resolution, and MS provides excellent selectivity and sensitivity. Previous work in our laboratory demonstrated that CE-MS techniques were quite useful for the metabolome analysis. We applied this approach to profile liver metabolites following acetaminophen-induced hepatotoxicity and revealed ophthalmate as a sensitive indicator of reduced glutathione (GSH) depletion. Our results specifically indicate that serum ophthalmate is a sensitive indicator of hepatic GSH depletion, and may be a new biomarker for oxidative stress.

• Access to Document (DOI)

Development of CE-MS for Meabolomics.

Soga, T.

J Mass Spectrom Soc Jpn 51 ( 3 ) 407 - 411 2003.06

Research paper (scientific journal), Single Work,  ISSN  1340-8097

　View Summary

Metabolomics, which can be defined as the measurement of the level of all intracellular metabolites, has become a powerful new tool for gaining insight into functional biology. Intercellular metabolites not only provide metabolic phenotypes but also inducers to gene expression. Thus, metabolome analysis will be as important as genome and proteome research. However, very few methods for a large-scale metabolite analysis have been reported. This paper reports a method for the direct and quantitative analysis of charged metabolite using capillary electrophoresis electrospray ionization mass spectrometry (CE-ESI-MS). Its utility is demonstrated in the determination of basal metabolic intermediates of glycolysis and the TCA cycle pathways in Bacillus subtilis cells, yielding new information about how changes in metabolites are related to sporulation events.

• Access to Document (DOI)

Simultaneous Determination of 8 Kinds of Organic Acids in Formula Feed by Capillary Electrophoresis.

Ishikuro, E., Hibino, H., Soga, T., Yanai, H., Sawada, H.

食品衛生学雑誌 41 ( 4 ) 261 - 267 2000.08

Research paper (scientific journal), Joint Work,  ISSN  0015-6426

　View Summary

配合飼料中の8種類の有機酸 (ギ酸, フマル酸, 酒石酸, リンゴ酸, クエン酸, 酢酸, 乳酸及びプロピオン酸) のキャピラリー電気泳動 (CE) による分析法を開発した. 各有機酸は水で同時抽出し, ろ過した後, ろ液を0.02mol/Lの水酸化ナトリウムで希釈し, 限外ろ過フィルターユニット (分画分子量30,000) で除タンパクを行いCEに供した. 3種類の配合飼料に8種類の有機酸を0.05～2.0%の範囲で添加して回収試験を行ったところ, 平均回収率は85.1～107.3%で繰り返し精度 (RSDr) は13.6%以内であった. また, 3試験室において共同試験を行った結果, 平均回収率は87.3～105.0%, 室間再現精度 (RSDR) は16.3%以内であった.

• Access to Document (DOI)